Since the invention of biological nutrient removal (BNR) technology, it has been widelyused in the filed of wastewater treatment. The study of microbial community andcharacteristics of BNR process could dramatically promote the development of wastewaterbiological treatment technology. This topic started with a preliminary analysis of the growthand BNR characteristics of the selected Pseudomonas sp. LY10. Then a group of specificmolecular probe for Pseudomonas was designed and it was applied in the dual-spec...