Tibet is a center of distribution and differentiation of genus Hordeum in China, and has a great deal of species resource. The sequences of the nontranscribed intergenic spacer (NTS) region of 5S nuclear ribosomal DNA was studied in 16 varieties of the wild relative of barley {Hordeum spontaneum) from different geographical range from Tibet of China. These sequences were determined by sequencing of PCR products clones. Alignment of sequences suggest that the 5S rDNA NTS contains two comparatively conserved ...