The genetic variation of the hybrid BC_1F_3 between Elymus dahuricus and Hordeum brevisubulatum were studied by means of biology, cytology and isozyme electrophoresis. The results were as follows:①The growing period, growth rate and regeneration of the hybrid BC_1F_3 tended toward recurrent parent H. brevisubulatum.②The fertilities of BC_1F_3 were recovered and enhanced in different degree.The rates of fertile pollen in YF_3-64 and YF_3-93 were 92.79% and 93.91% respectively, the natural seed settin...