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利用荧光AFLP标记分析江西省香果树的遗传多样性

Genetic Diversity Analysis of Emmenopterys henryi in Jiangxi Province by Fluorescent AFLP Markers

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【作者】 牛艳丽; 彭焱松; 胡菀; 冯晨;

【Author】 NIU Yanli;PENG Yansong;HU Wan;FENG Chen;Jiangxi Provincial Key Laboratory of ex Situ Plant Conservation and Utilization,Lushan Botanical Garden,Chinese Academy of Sciences;

【机构】 江西省中国科学院庐山植物园,植物迁地保护与利用江西省重点实验室;

【摘要】 香果树是一种珍稀濒危落叶树种,具有木材、药用、园林绿化和化工等价值,但由于过度采伐、旅游业和生态环境污染等原因,目前分布区已破碎化、缩小化。本研究运用AFLP标记对江西省4个香果树居群64份材料进行遗传多样性分析。结果表明,香果树的多态位点百分率为91.37%,Nei.s基因多样性指数和Shannon.s信息指数分别为0.2731和0.4166,基因流为3.8028,表明4个群体的遗传多样性水平较高,且相互间有基因交流。居群水平上,大围山居群(DWS)的Nei.s基因多样性和Shannon.s信息指数最高,其遗传多样性最丰富。AMOVA分析表明,遗传变异主要存在于群体内;UPGMA聚类分析发现,除武夷山居群(WYS)外,其他样品在地理位置上没有明显的类群分化; STRUCTURE和PCA聚类分析结果表明,4个群体均被划分为3个类群。本研究结果可为江西省香果树的保护提供重要的参考依据。

【Abstract】 Emmenopterys henryi is a rare and endangered deciduous species valuedfor wood,medical,landscaping and chemical applications. Unfortunately,the current distribution areas of E.henryi are fragmented and have diminished,due to overlogging,tourism and pollution on the ecological habitat. In thisstudy,we sampled 64 individuals from four geographical populations in Jiangxi Province and analyzed their genetic diversity by using fluorescent AFLP markers. The percentage of polymorphic loci of E.henryiwas 91. 37%. The Nei.s gene diversity index(H)and Shannon.s information index(I)were 0. 2731 and 0. 4166,respectively. The gene flow was 3. 8028. These indicated high genetic diversity and genetic exchanges among populations. At the population level,the Daweishan population(DWS)represented the highest diversity. AMOVA results showed that genetic variation mainly existed within population. The cluster analysis by UPGMA showed clear geographical grouping except for Wuyishan population(WYS). Notably,both STRUCTURE and PCA analyses consistently divided four populations into three genetic groups. These findings can provide an important reference for the effective protection of E.henryiin Jiangxi Province.

【基金】 中国科学院战略生物资源计划植物种质资源创新平台项目(KFJ-BRP-007-013)
  • 【会议录名称】 中国植物园(第二十八期)
  • 【会议名称】2025年中国植物园学术年会
  • 【会议时间】2025-11-11
  • 【会议地点】中国广西桂林
  • 【分类号】S792.99
  • 【主办单位】中国植物学会
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