【作者】 张颖； 叶青华； 曹璐璐； 牛沁雅； 陈诺； 段子璇； 陈谋通； 李滢； 吴清平；

【Author】 Zhang Ying;Ye Qinghua;Cao Lulu;Niu Qinya;Chen Nuo;Duan Zixuan;Chen Moutong;Li Ying;Wu Qingping;Institute of Microbiology,Guangdong Academy of Sciences;College of Agriculture and Biology,Shanghai Jiao Tong University;

【机构】 广东省科学院微生物研究所； 上海交通大学农业与生物学院；

【摘要】 人体微生物群的紊乱影响着多种疾病的发生与发展。治疗用活菌凭借其遗传可操作性、厌氧特性及免疫调节功能,正逐渐成为一类具有广阔前景的治疗制剂。然而,其在临床应用过程中仍面临活性维持与精准时空释放的挑战,尤其是在恶劣的胃肠道环境中。本综述重点总结了借助物理化学与生物技术进展所开发的创新型细菌功能化封装策略,全面探讨了如何通过细菌封装技术为活微生物提供物理屏障并增强治疗用活菌的黏附能力,同时赋予活菌更优越的材料特性。此外,本文还概述了细菌表面涂层如何促进活菌的靶向递送与精准时空释放,阐明了该策略在治疗不同疾病方面的潜在应用,并对临床转化中的关键挑战提出重要观点。本综述系统分析了功能化细菌封装与创新生物医学应用之间的关联,为开发新一代细菌治疗策略提供理论参考。更多还原

【Abstract】 The disturbance of the human microbiota influences the occurrence and progression of many diseases.Live therapeutic bacteria,with their genetic manipulability,anaerobic tendencies,and immunomodulatory properties,are emerging as promising therapeutic agents.However,their clinical applications face challenges in maintaining activity and achieving precise spatiotemporal release,particularly in the harsh gastrointestinal environment.This review highlights the innovative bacterial functionalized encapsulation strategies developed through advances in physicochemical and biological techniques.We comprehensively review how bacterial encapsulation strategies can be used to provide physical barriers and enhanced adhesion properties to live microorganisms,while introducing superior material properties to live bacteria.In addition,this review outlines how bacterial surface coating can facilitate targeted delivery and precise spatiotemporal release of live bacteria.Furthermore,it elucidates their potential applications for treating different diseases,along with critical perspectives on challenges in clinical translation.This review comprehensively analyzes the connection between functionalized bacterial encapsulation and innovative biomedical applications,providing a theoretical reference for the development of next-generation bacterial therapies.更多还原