【作者】 苏昊； 徐振林； 王弘； 温棚；

【Author】 Su Hao;Xu Zhenlin;Wang Hong;Wen Peng;College of Food Science,South China Agricultural University;

【机构】 华南农业大学食品学院；

【摘要】 草甘膦是一种广泛使用的除草剂。人体摄入后可能造成皮肤刺激,呼吸系统不适等危害。因此,亟需开发一种用于草甘膦快速检测的方法。考虑到草甘膦的危害和现有检测方法的局限性,本文构建了一种将比率荧光探针CuAl-LDH@\[Ru（bpy）₃\]²⁺固定在电纺纳米纤维膜中的新型传感平台,用于快速选择性检测草甘膦。首先,通过水热合成法合成了具有花瓣状形貌的CuAl-LDH,并对其化学结构组成进行了表征。随后以CuAl-LDH作为响应信号,\[Ru（bpy）₃\]²⁺作为参考信号构建比率式荧光探针,并对其优化条件进行了研究。在优化的检测条件下,比率荧光探针在0～600μmol/L的浓度范围内呈线性响应（R²=0.9931）,检出限（LOD）为0.41μmol/L。在实际样品添加回收实验中加标回收率为98.70%～110.32%,相对标准偏差小于5.86%。此外,将CuAl-LDH@\[Ru（bpy）₃\]²⁺负载在电纺纳米纤维膜上,结合智能手机对颜色的快速识别能力,开发了一种集成智能手机的便携式电纺纳米纤维膜检测平台。该检测平台可实现草甘膦的现场、灵敏检测（LOD=0.83μmol/L）。本研究为环境监测和食品安全评估提供了一种可靠、高效和实用的工具。更多还原

【Abstract】 Glyphosate was a widely used herbicide.After human ingestion,it might cause hazards such as skin irritation and respiratory discomfort.Therefore,the urgent development of a method for the rapid detection of glyphosate was required.Considering the hazards of glyphosate and the limitations of existing detection methods,a novel sensing platform based on ratiometric fluorescent probes(CuAl-LDH@\ [Ru（bpy）₃\]²⁺) immobilized on electrospun nanofiber membranes was constructed in this study for the rapid and selective detection of glyphosate.Firstly,CuAl-LDH with a petal-like morphology was synthesized by hydrothermal synthesis method,and its chemical structure and composition were characterized.Subsequently,a ratiometric fluorescent probe was constructed using CuAl-LDH as the response signal and \ [Ru（bpy）₃\]²⁺ as the reference signal,and the optimization conditions for this probe were investigated.Under optimal detection conditions,the probe showed a linear response over the concentration range of 0-600 μmol/L（R²=0.9931）,with a detection limit（LOD） of 0.41 μmol/L.In the spiked recovery experiments of real samples,the spiked recovery rates ranged from 98.70% to 110.32%,and the relative standard deviations（RSDs） were less than 5.86%.In addition,CuAl-LDH@\[Ru（bpy）₃\]²⁺ was loaded onto electrospun nanofiber membranes.Combined with the rapid color recognition capability of smartphones,a portable electrospun nanofiber membrane detection platform integrated with a smartphone was developed.This detection platform could achieve on-site and sensitive detection of glyphosate（LOD=0.83 μmol/L）.This study provided a reliable,efficient,and practical tool for environmental monitoring and food safety assessment.更多还原