【作者】 宋紫薇； 陈婷； 沈梅红；

【Author】 Song Ziwei;CHEN Ting;SHEN Meihong;College of Acupuncture-Moxibustion and Tuina,College of Health and Rehabilitation,Nanjing University of Chinese Medicine;Jiangsu Province Hospital of Shengze;

【机构】 南京中医药大学针灸推拿学院·养生康复学院； 江苏省盛泽医院；

【摘要】 目的观察电针对雷公藤多苷诱导的POI大鼠卵巢功能的保护作用及TGF-β1/Smads信号通路的影响。方法选取27只动情周期正常的雌性SD大鼠随机分为空白组、模型组、电针组,每组9只。干预结束后,计算动情周期紊乱率,观察各组大鼠卵巢组织学形态;检测血清卵泡刺激素（FSH）、雌二醇（E₂）含量及大鼠卵巢组织中TGF-β1、Smad2、Smad7蛋白及基因表达水平。结果与模型组相比,电针组大鼠卵巢指数升高,动情周期紊乱率降低,原始卵泡、窦卵泡数量增加,闭锁卵泡减少,血清FSH含量降低,E₂含量升高,卵巢组织中TGF-β1、Smad2蛋白及mRNA表达增多,Smad7蛋白及mRNA表达减少（P<0.05）。结论电针能够明显改善POI大鼠的卵巢功能,其作用机制可能与调控TGF-β1/Smads信号通路有关。更多还原

【Abstract】 Objective To observe the protective effect of electroacupuncture（EA） on ovarian function and TGF-β1/Smads signaling pathway in rats with premature ovarian insufficiency（POI） induced by tripterygium wilfordii polyglycosides.Methods Twenty-seven female Sprague-Dawley rats with normal estrous cycle were randomly divided into control,model and electroacupuncture groups,with 9 rats in each group.At the end of the intervention,the rate of estrous cycle disorders was calculated,and the histological morphology of the ovaries was observed;serum follicle-stimulating hormone（FSH） and estradiol（E₂） levels and the levels of TGF-β1,Smad2 and Smad7 protein and gene expression in rat ovarian tissues were detected.Results Compared with the model group,the ovarian index of the rats in the electro-acupuncture group was increased,and the motility cycle disorder rate decreased,the number of primordial follicles,sinus follicles increased,atretic follicles decreased,serum FSH content decreased,E₂ level increased,and the expression of TGF-β1 and Smad2 proteins and m RNAs increased in the ovarian tissues,and Smad7 protein and m RNA expression decreased（P<0.05）.Conclusion Electroacupuncture can significantly improve ovarian function in POI rats,and its mechanism of action may be related to the regulation of TGF-β1/Smads signaling pathway.更多还原