【作者】 郝胜杰； 李欠； 陈垣； 马靖捷； 魏滟芝； 王冰洁； 胡芳弟； 曹嘉琪； 吴佩润； 薛智元；

【机构】 干旱作物学国家重点实验室； 甘肃农业大学； 兰州大学；

【摘要】 背景:神经官能症是常见的心理健康问题,与神经发育和神经递质的异常有关。柴胡疏肝散(XYS)被广泛运用于中医临床,被认为具有调节情绪和神经功能的潜力。本研究旨在探讨XYS对神经官能症模型小鼠的治疗效果以及其可能的作用机制。材料与方法:采用慢性不可逆性应激(CUMS)和苯巴比妥诱导的神经官能症模型小鼠。小鼠每天口服XYS溶液,连续21天。通过行为学测试,包括强迫游泳实验(FST)、开放田间测试(OFT)、旷场试验(EPM)等,评估小鼠的情绪状态和神经功能。同时采用免疫组化检测海马和杏仁核中的相关指标,包括小胶质细胞标志物Iba-1的表达。结果:与模型组相比,XYS处理组小鼠在FST中表现出更长的游泳时间,OFT和EPM中显示更多的探索活动,提示XYS对抑郁症和神经官能症有治疗效果。免疫组化结果显示,XYS能够减轻CUMS和苯巴比妥引起的小鼠海马和杏仁核中Iba-1阳性细胞的增加,其可能通过抑制小胶质细胞激活来发挥作用。结论:柴胡疏肝散表现出对神经官能症的治疗潜力,可能通过调节海马和杏仁核中小胶质细胞的激活来实现。这一发现为中医药在神经精神疾病治疗中的应用提供了新的证据,同时为深入理解柴胡疏肝散的作用机制提供了基础。更多还原

【Abstract】 Background:Neurofunctional disorders are common mental health issu es associated with abnormal neural development and neurotransmitter dysfunctio n.Chaihu Shugan San(XYS),widely used in traditional Chinese medicine,is believed to have the potential to regulate mood and neural function.This study aims to investigate the therapeutic effects of XYS on a neurofunctional disord er mouse model and its possible mechanisms.Materials and Methods:A neurofu nctional disorder mouse model was induced using chronic unpredictable mild st ress(CUMS) and pentobarbital.Mice were orally administered XYS solution d aily for 21 days.Behavioral tests,including forced swimming test(FST),open field test(OFT),and elevated plus-maze(EPM),were conducted to assess the emotional state and neural function of the mice.Immunohistochemistry was e mployed to detect relevant markers,including Iba-1 expression in the hippocam pus and amygdala.Results:Compared to the model group,mice in the XYS tre atment group exhibited longer swimming time in the FST and showed more ex ploratory activity in the OFT and EPM,suggesting a therapeutic effect of XYS on depression and neurofunctional disorders.Immunohistochemistry results sho wed that XYS could alleviate the increase of Iba-1 positive cells in the hippoc ampus and amygdala induced by CUMS and pentobarbital,possibly by inhibiti ng the activation of microglial cells.Conclusion:Chaihu Shugan San demonstrat es potential therapeutic effects on neurofunctional disorders,possibly by modula ting the activation of microglial cells in the hippocampus and amygdala.This f inding provides new evidence for the application of traditional Chinese medicin e in the treatment of neuromental disorders and lays the foundation for a deep er understanding of the mechanisms of Chaihu Shugan San.更多还原