【作者】 吴金姗； 刘回民； 郑明珠； 刘美宏； 蔡丹； 刘景圣；

【Author】 Wu Jinshan;Liu Huimin;Zheng Mingzhu;Liu Meihong;Cai Dan;Liu Jingsheng;College of Food Science and Engineering,Jilin Agricultural University;National Engineering Research Center for Deep Processing of Wheat and Corn;

【机构】 吉林农业大学食品科学与工程学院； 小麦和玉米深加工国家工程研究中心；

【摘要】 玉米黄素、虾青素等类胡萝卜素具有强抗氧化活性,研究发现其可作用于线粒体发挥多种生理活性,但其不良稳定性和低生物利用度限制了在食品工业中的应用。本研究以大豆卵磷脂和胆固醇为原料,采用薄膜分散-超声法制备纳米脂质体,并基于酯化反应制备β-葡聚糖和三苯基溴化膦复合物(BG-TPP),利用其修饰纳米脂质体(BG-TPP@Lipo),实现类胡萝卜素的线粒体靶向递送。结果表明,BG-TPP@Lipo的最佳制备条件为:卵磷脂与胆固醇质量比4:1,卵磷脂与类胡萝卜素质量比100:1,BG-TPP添加量0.3%,240W功率下超声6min,所得脂质体平均粒径为131.96 nm,PDI为0.202,Zeta电位为-33.67mV,包封率为83.7%,TEM图像呈现均匀的单分散球形。体外模拟胃肠消化结果证明,BG-TPP@Lipo封装的类胡萝卜素释放率<40%,显著低于游离类胡萝卜素(>80%),表明BG-TPP修饰有利于抵抗胃肠道的消化降解。体外试验表明,BG-TPP@Lipo能够特异性聚集在细胞线粒体中。综上,BG-TPP@Lipo在提高类胡萝卜素的生物利用度及线粒体靶向递送方面具有良好潜力。更多还原

【Abstract】 Carotenoids such as zeaxanthin and astaxanthin have strong antioxidant activity and have been found to act on mitochondria to exert a variety of physiological activities.However,their poor stability and low bioavailability limit their application in the food industry.In this study,soy lecithin and cholesterol were used to prepare nanoliposomes by film-dispersion combined with ultrasonication.Besides,β-glucan and triphenylphosphonium bromide complex(BG-TPP) were prepared based on the esterification reaction,which was utilized to modify the nanoliposomes(BG-TPP@Lipo) to achieve mitochondriatargeted delivery of carotenoids.The results showed that the optimal conditions for the preparation of BG-TPP@Lipo were as follows:the mass ratio of lecithin and cholesterol was 4:1,the mass ratio of lecithin and carotenoid was 100:1,the addition of BG-TPP was 0.3%,and sonicate for 6 min at 240 W.The average particle size of mitochondria-targeting nanoliposomes is 131.96 nm,the PDI is 0.202,the Zeta potential is-33.67 mV,and the encapsulation efficiency is 83.7%.The TEM images were shown as homogeneous nanospheres.The in vitro simulated gastrointestinal digestion results demonstrated that the carotenoid release rate of BG-TPP@Lipo was less than 40 %,which was significantly lower than that of free carotenoids(more than 80 %),suggesting that the BG-TPP modification was beneficial in resisting the digestive degradation in the gastrointestinal tract.In vitro assays showed that BG-TPP@Lipo was able to specifically aggregate in cellular mitochondria.In conclusion,BG-TPP@Lipo showed good potential in improving the bioavailability of carotenoids and achieving mitochondria-targeted delivery.更多还原