【作者】 王丽鸳； 韦康； 张成才； 成浩；

【Author】 WANG Li-yuan;WEI Kang;ZHANG Cheng-cai;CHENG Hao;Tea Research Institute,Chinese Academy of Agricultural Sciences,National Center for Tea Improvement;

【机构】 中国农业科学院茶叶研究所国家茶树改良中心；

【摘要】 利用Perl语言,对茶树花转录组序列进行大通量SSR位点的发掘,发现含不同重复基元SSRs的序列有10,290条,共12,582个SSRs,平均2.41 kb出现一个SSR。在茶树花的转录组序列中共发现340种碱基重复模式,所占比例最高的是(AG/CT) n (44.99%)。在49586条注释成功的茶树花的unigene中,共发现了10,490个SSR位点,其中位于编码区的SSR位点数仅为1,917个,基因编码区SSR的出现频率仅为0.102 SSR/1000 bp,而在非编码区SSR的出现频率为3.072 SSR/1000 bp。在基因编码区中出现频率最高的是三碱基重复类型的SSR(1140,59.5%),其次是六碱基重复(524,27.3%)。茶树转录组所含微卫星序列长度的分布,严重偏离正态分布,以重复长度小于15bp的SSR短重复序列最多,大于30个bp的较长序列重复仅为3.6%。茶树花转录组中,含微卫星序列基因的整体表达水平要显著低于不含有微卫星序列基因的表达水平,含复杂微卫星序列的基因整体表达水平是最低。更多还原

【Abstract】 The microsatellites or simple sequence repeats(SSRs) in the Camellia sinensis floral transcriptome were characterized.In total 12,582 SSRs were identified in 10,290 unigenes,scanning one SSR per every2.41 kb.Among,all 340 SSR motifs,(AG/CT) n were the most frequent repeat motif(44.99%).A total of10409 SSRs occurred in 49586 unigenes with BLAST matches to annotated proteins in four databases,of which only 1 917 occurred in protein-coding regions of these sequences.The density of SSRs was much higher in non-coding regions that followed coding regions than in coding regions(0.102 SSRs per 1000 basepairs in coding regions vs.3.072 in non-coding regions).Of the six repeat motifs,tri-nucleotide repeats were the most abundant in coding regions(1140),followed by hexa-(524) repeats.The length distribution of SSRs was seriously deviated from the normal distribution.The microsatellites with length below 15 bp were in maximum proportion,while the microsatellites over 30 bp were only 3.6%.The expression level of genes containing microsatellites was significantly less than that of genes containing non-microsatellites.The overall expression levels of genes containing compound microsatellites were lowest.更多还原