【作者】 韩艳娜； 张卫； 彭程；

【Author】 Han yanna;Zhang wei;Peng cheng;East China University of Science and Technology, School of Resources and Environmental Engineering;

【机构】 华东理工大学资源与环境工程学院；

【摘要】 近年来,十溴二苯乙烷（DBDPE）作为最主要的新型溴系阻燃剂（NBFRs）,被广泛应用于工业制造中。而塑料制品的大量使用和回收不可避免地引起了DBDPE和微塑料（MPs）的环境释放,并在土壤中累积及共存。然而,比较以聚乙烯（PE）、聚丙烯（PP）为代表的传统化石基塑料和以聚乳酸（PLA）为主的新型生物基塑料介导下DBDPE的生物富集及毒性效应仍研究尚浅。本研究通过模拟10 mg kg^-1 DBDPE单一暴露和10 mg kg-1DBDPE-MPs联合暴露（PLA、PP和PE）模式,探讨不同典型MPs介导下DBDPE在土壤-蚯蚓体系中的生物富集、神经毒性、组织损伤和基因调控。结果显示,与神经毒性相关的生物标志物明显异常,包括乙酰胆碱酯酶、Na⁺/K⁺-ATP酶、Ca²⁺/Mg²⁺-ATP酶、羧酸酯酶的活性及钙和谷氨酸含量。DBDPE-MPs共暴露引起了蚯蚓更严重的表皮损伤。转录组学分析显示,联合暴露下蚯蚓的差异表达基因（DEGs）主要与炎症、免疫系统、消化系统、内分泌系统和代谢有关。与DBDPE单一暴露相比,三种MPs均显著加强了DBDPE对蚯蚓的胁迫效应,尤其是PE和PLA。它们进一步影响了DBDPE胁迫蚯蚓的内分泌系统和信号传导。对比DBDPE单一暴露,仅在DBDPE-MPs联合暴露中特有的DEGs表明,蚯蚓的解毒系统被显著激活。本研究发现MPs加剧了DBDPE胁迫蚯蚓的神经系统、表皮损伤和基因调控,有助于评估土壤中塑料废弃物的生态风险。更多还原

【Abstract】 In recent years, decabromodiphenyl ethane（DBDPE） has been widely used in industrial manufacturing as the most prominent new brominated flame retardant（NBFR）. extensive use of plastic products and recycling inevitably cause environmental releases of DBDPE and microplastics（MPs）, which accumulate and co-exist in soil. However, a comparison of the bioconcentration and toxicity effects of DBDPEs mediated by traditional fossil-based plastics such as polyethylene（PE） and polypropylene（PP） and popular bio-based plastics such as polylactic acid（PLA） is still poorly studied. In this study, we investigated the bioconcentration, neurotoxicity,tissue damage, and gene regulation of DBDPE in a soil-earthworm system mediated by different typical MPs by simulating 10 mg kg^-1 DBDPE single exposure and 10 mg kg^-1 DBDPE-MPs combined exposure（PLA, PP, and PE）. The results showed significant abnormalities in biomarkers associated with neurotoxicity, including acetylcholinesterase, Na⁺/K⁺-ATPase,Ca²⁺/Mg²⁺-ATPase, carboxylesterase activity, and calcium and glutamate levels. DBDPE and MP co-exposure caused more severe epidermal damage in earthworms. Transcriptomic analysis revealed that differentially expressed genes（DEGs） in earthworms under combined exposure were mainly associated with inflammation, immune, digestive, endocrine, and metabolism. All three MPs significantly enhanced the stressful effects of DBDPE on earthworms compared to single DBDPE exposure, especially PE and PLA, which further affected the endocrine system and signaling in DBDPE-stressed earthworms. In contrast to single DBDPE exposure, DEGs that were specific only to combined DBDPE-MPs exposure indicated that the detoxification system of earthworms was significantly activated. The present study found that MPs exacerbated neurological and epidermal damage and gene regulation in DBDPE-stressed earthworms,contributing to the assessment of the ecological risk of plastic waste in the soil.更多还原