【作者】 田宝明； 耿艳； 蔡铭； 孙培龙； 叶帮伟； 杨开；

【Author】 TIAN Baoming;GENG Yan;CAI Ming;SUN Peilong;YE Bangwei;YANG Kai;College of Food Science and Technology, Zhejiang University of Technology;Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology),China National Light Industry;Zhejiang Wise-plus Health Technology Co., Ltd;

【机构】 浙江工业大学食品科学与工程学院； 中国轻工业食品大分子资源加工技术重点实验室； 浙江慧和健康科技有限公司；

【摘要】 以碱法提取灰树花加工渣,获得灰树花碱提多糖(Grifola frondosa alkaline polysaccharide, GFAP),分析其理化性质,探究体外消化及发酵时的变化规律,以及对肥胖人群肠道菌群调节作用;研究GFAP对高脂膳食(High-fat deits,HFD)诱导肥胖小鼠的改善和肠道菌群调节作用,并通过粪菌移植(FMT)验证肠道菌群的作用。结果表明:GFAP中多糖和β-葡聚糖含量分别为89.29%和60.57%,主要由葡萄糖组成;不易被消化液中不被降解,可被肠道菌群分解利用,增加短链脂肪酸含量,降低体系p H值;显著改善HFD诱导引起的胰岛素抵抗和糖耐量异常,改善脂质代谢紊乱,显著降低HFD诱导引起的SREBP-1c、PPARγ和ACC等肝脏中脂质积累基因表达升高,显著增加PGC-1α和PPARα等脂质分解基因的表达,增加肝脏中AMPK的磷酸化水平,降低FAS、ACC及SREBP-1c脂质积累蛋白的表达。GFAP的摄入能够提高小鼠肠道菌群的物种多样性和丰度,改变肠道菌群的结构。FMT研究发现,GFP通过肠道菌群对HFD诱导小鼠起到抑制肥胖的作用。研究结果为灰树花减肥功能食品开发提供科学依据。更多还原

【Abstract】 Grifola frondosa alkali-extracted polysaccharide(GFAP) was obtained by alkaline extraction of Grifola frondosa processing residue. The physicochemical properties, changes of in vitro digestion and fermentation, and the regulatory effects of GFAP on gut microbiota of obese people were investigated. The improvement of symptoms and the regulation of intestinal flora in high-fat induced obese mice by GFAP were studied, and the role of intestinal flora was verified by fecal microbiota transplantation(FMT). The results showed that the contents of polysaccharide and β-glucan in GFAP were 89.29%and 60.57%, respectively, which were mainly composed of glucose. GFAP was not easy to be degraded in digestive juices,and can be decomposed and utilized by intestinal flora to increase the content of short-chain fatty acids and reduce the pH value of the system. GFAP intake significantly reduced the HFD-induced increase in the expression of lipid accumulation genes, such as SREBP-1c, PPARγ, and ACC, and significantly increased the expression of lipolytic genes such as PGC-1αand PPARα. The phosphorylation level of AMPK was increased, and the expression levels of FAS, ACC and SREBP-1c lipid accumulation protein were decreased. The uptake of GFP could increase the diversity and abundance of gut microbiota, and alter the structure of gut microbiota. The results of FMT discovered that GFAP inhibited obesity in HFD-induced mice through intestinal flora. Taken together, our findings might provide scientific basis for the development of anti-obesity functional foods containing Grifola frondosa.更多还原