【作者】 余天； 谢依婷； 王中亮； 黎晶晶； 申云鹏； 袁娟丽； 高金燕； 武涌； 陈红兵；

【Author】 Yu Tian;Xie Yiting;Wang Zhongliang;Li Jingjing;Shen Yunpeng;Yuan Juanli;Gao Jinyan;Wu Yong;Chen Hongbing;State Key Laboratory of Food Science and Resources,Nanchang University;Sino German Joint Research Institute,Nanchang University;School of Food Science and Technology,Nanchang University;School of Pharmaceutical Science,Nanchang University;

【机构】 南昌大学食品科学与资源挖掘全国重点实验室； 南昌大学中德联合研究院； 南昌大学食品学院； 南昌大学药学院；

【摘要】 乳糜泻是一种慢性炎症性的自身免疫性小肠疾病,全球约有1%的人患有乳糜泻。迄今为止,患者终生严格遵守无麸质饮食是乳糜泻唯一且最有效的治疗方案,但这不仅难以坚持,还会导致新的营养失衡,因此迫切需要寻找新的营养干预措施。我们旨在研究槲皮素的营养干预对乳糜泻的影响。本研究利用已建立的麸质蛋白所诱导的乳糜泻体内外模型,系统评估了槲皮素对乳糜泻肠道氧化损伤、免疫反应、炎症损伤和肠道微生物菌失衡的调节作用。我们发现,槲皮素能对乳糜泻的发病发挥干预作用,这不仅是因为它本身所具备的抗氧化特性,更由于它能调控免疫细胞的功能和肠道微生物群的结构,特别是对Th1/Th2/Treg免疫细胞亚群及其功能的调节、对乳糜泻标志菌群(如Clostridium_celatum和Bacteroides_acidifaciens)丰度的抑制以及对有益微生物(如Butyricoccus_pullicaecorum和Bifidobacterium_longum)数量的上调,这些最终共同改善麸质蛋白所诱导的乳糜泻相关肠道炎症。更多还原

【Abstract】 Celiac disease is a chronic inflammatory autoimmune disease of the small intestine,and approximately 1% of the world’s population is afflicted with celiac disease.The most effective treatment option to date is that the patient is required to strictly follow the gluten-free diet for the whole life,but it’s difficult to be adhered to and can lead to new nutritional imbalances,making it urgent to find novel nutritional interventions.We aimed to investigate the effects of nutritional intervention with quercetin on celiac disease.This study systematically assessed the regulatory roles of quercetin on intestinal oxidative damage,immune response,inflammatory damage and intestinal microflora dysbiosis in celiac disease by utilizing the established celiac in vitro and in vivo models induced by gluten.We discovered that quercetin could perform the critical role in intervening in the pathogenesis of celiac disease,not only owing to its own antioxidant properties,but also because of its modulation of immune cell function and intestinal microflora structure,particularly on the regulation of Th1/Th2/Treg immune cell subpopulations and their functions,the inhibition of the abundance of celiac disease marker flora such as Clostridium_celatum and Bacteroides_acidifaciens,and upregulation of the abundance of beneficial bacteria such as Butyricoccus_pullicaecorum and Bifidobacterium_longum,which ultimately worked together to ameliorate the celiac-related intestinal inflammation triggered by gluten.更多还原