【作者】 孙钰薇； 刘志佳； 胡小松； 易俊洁；

【Author】 Sun Yuwei;Liu Zhijia;Hu Xiaosong;Yi Junjie;Faculty of Food Science and Engineering,Kunming University of Science and Technology;College of Food Science and Nutritional Engineering,China Agricultural University;

【机构】 昆明理工大学食品科学与工程学院； 中国农业大学食品科学与营养工程学院；

【摘要】 植物乳杆菌(Lactiplantibacillus plantarum)是一种常见于发酵制品中的乳酸菌,对食品工业和人体健康有着多重有益的功效。缺乏快速有效的方法评估菌株的益生功能特性严重制约了该菌株的应用。在本研究中,通过体外实验评估了从中国传统发酵食品中分离的植物乳杆菌的益生性,包括胃酸和胆盐耐受性、细胞粘附能力、模拟人体胃肠道环境中的存活率、抗氧化活性、胆盐水解酶活性和抗菌活性。所有受试菌株表现出较高的抗氧化能力、胆盐水解酶活性和抗菌活性,这可能是植物乳杆菌的共同特征。在这些菌株中,B652、C232、D444和E932被确定为综合益生特性最好的菌株,对它们进行全基因组测序,解析与功能相关的关键调控基因。结果表明,菌株抗氧化活性、粘附活性和在模拟胃肠环境中的存活能力分别与其抗氧化酶编码基因、细胞表面蛋白编码基因和应激反应调控基因数量和种类密切相关。通过菌株的体外益生表型和基因组特征关联综合分析,可为评估和鉴定具有益生功能特性的菌株提供参考。更多还原

【Abstract】 Lactiplantibacillus plantarum,a lactic acid bacteria commonly found in fermented products,has multiple beneficial effects on the food industry and human health.The application of this type of strains has been limited by the lack of rapid and effective approaches for the evaluation of probiotic properties of strains.In the present research,the probiotic potential of Lactiplantibacillus plantarum strains isolated from different Chinese traditional fermented food were evaluated,including acid and bile salt resistance,adhesion ability,survival in simulated human gastrointestinal transit,antioxidant activity,bile salt hydrolase activity,and antibacterial activity.All the tested strains showed a high antioxidant capacity,BSH activity,and antibacterial activity,which might be the common characteristics of Lactiplantibacillus plantarum.Among these strains,B652,C232,D444,and E932 were identified as the best performed strains and selected for the whole genome sequencing to resolve key regulatory genes related to function.Results demonstrated that the antioxidant activity,adhesion activity,and ability to survive in the simulated gastric environment were found to be closely correlated with numbers and types of antioxidant enzyme encoding genes,cell-surface proteins encoding genes,and stress response genes,respectively.The outcome of the study could be helpful to link the probiotic phenotype and genomic characteristics of potential strains,thereby providing knowledge and references to discover and evaluate probiotic candidates.更多还原