【作者】 闫琳琳； 张红； 张志民；

【Author】 Yan Linlin;Zhang Hong;Zhang Zhimin;Department of Endodontics, Hospital of Stomatology, Jilin University;

【机构】 吉林大学口腔医院牙体牙髓病科；

【摘要】 目的:研究锌/氮掺杂二氧化钛纳米粒子结合LED光固化灯协同抑制变异链球菌的实验效果。方法:1)合成锌/氮掺杂二氧化钛纳米粒子(Zn-NTiO2NPs):设计适当比例,采用溶胶-水热法合成Zn-N-TiO2NPs。2)表征:通过X射线衍射(X-ray diffraction,XRD)、扫描电子显微镜(scanning electron microscope,SEM)、UV-vis吸收光谱对Zn-N-TiO2NPs进行表征。3)抗浮游菌活性试验:在96孔板中配置浓度分别为1.0mg/ml、2.0mg/ml、4.0mg/ml Zn-NTiO2NPs稀释液50 L,每孔加入50 L菌悬液(107CFU/ml),使药物菌悬液终浓度分别为0.5mg/mL、1.0mg/mL、2.0mg/mL,分别用LED光固化灯照射5min,37℃厌氧培养24小时后,梯度稀释药物菌悬液,分别取100 L,均匀涂布于BHI琼脂平板,37℃厌氧培养48小时,进行菌落计数。结果:1)采用溶胶-水热法成功合成了Zn-N-TiO2NPs。2)XRD结果显示成功合成Zn-N-TiO2NPs;SEM结果显示样品呈较为规则的球状结构,形态较为均匀;通过UV-vis吸收光谱观察Zn-N-TiO2NPs的可见光吸收强度与纯二氧化钛相比变强。3)浓度为2.0mg/ml,LED光照时间为5min实验组的抗菌率可达98.87%。结论:锌/氮掺杂二氧化钛纳米粒子结合LED光可抑制变异链球菌。更多还原

【Abstract】 Objectives:To study the synergistic inhibitory effect of Zinc/nitrogen doped titanium dioxide nanoparticles combined with LED light curing lamp on Streptococcus mutans.Methods:1) Synthesis of Zinc/nitrogen doped titanium dioxide nanoparticles（Zn-N-TiO₂ NPs）:Synthesis of Zn-N-TiO₂ NPs by sol-hydrothermal method with appropriate proportion design.2) Characterization:Zn-TiO₂ NPs were characterized by X-ray diffraction（XRD）,scanning electron microscope（SEM） and UV-VIS absorption spectrum.3) Anti-planktonic bacteria activity test:Set the concentrations of Zn-N-TiO₂ NPS diluted 50 L in the 96-well plate at 1.Omg/mL,2.0 mg/mL,and 4.0 mg/mL,and add 50 L bacterial suspension（10⁷ CFU/mL） to each well.The final concentration of the suspension was 0.5 mg/mL,1.Omg/mL and2.Omg/mL,and the suspension was irirated with LED light curing lamp for 5 min.After anaerobic culture at 37℃ for 24 hours,the gradient dilution of the suspension was taken,and 100 L was evenly coated on BHI agar plate,and anaerobic culture at 37℃for 48 hours.Colony count was performed.Results:1) Zn-TiO₂ NPs was successfully synthesized by sol-hydrothermal method.2) XRD results show that Zn-TiO2 NPs was synthesized successfully;SEM results show that the samples have regular spherical structure and uniform morphology.The visible light absorption intensity of Zn-NTiO₂ NPs was stronger than that of pure titanium dioxide by UV-vis absorption spectrum.3) The antibacterial rate of the experimental group was 98.87% at 2.Omg/ml and 5 min of illumination.Conclusion:Zinc/nitrogen doped titanium dioxide nanoparticles combined with LED light can inhibit Streptococcus mutans.更多还原