【作者】 何璇； 孟紫君； 陈文霞；

【Author】 He Xuan;Meng Zijun;Chen Wenxia;Conservative Dentistry & Endodontics Department, College and Hospital of Stomatology, Guangxi Medical University;

【机构】 广西医科大学附属口腔医院牙体牙髓科；

【摘要】 目的:探讨不同浓度的氯化钴(CoCl2)诱导的化学缺氧对人牙髓干细胞(hDPSCs)促血管生成潜能的影响。方法:体外分离培养hDPSCs,经表面标志物检测鉴定后,使用不同浓度的(0.05mM、0.1 mM、0.2mM、0.5mM、1.0mM) CoCl2构建体外细胞缺氧模型。通过CCK-8、细胞迁移实验检测缺氧对hDPSCs增殖、迁移的影响;通过RT-PCR检测细胞内has-miR-143-5p、HIF-1α、VEGF的mRNA表达水平。结果:含不同浓度CoCl2条件培养基处理48 h后,与对照组相比,0.05 mM与0.1 mM CoCl2可以促进hDPSCs的增殖,而0.5 mM与1.0mMCoCl2则抑制其增殖;0.05mM、0.1 mM、0.2mM、0.5mMCoCl2对hDPSCs迁移有促进的作用;HIF-1α、VEGF的mRNA表达水平随着CoCl2浓度的升高而升高,而细胞分化调控因子has-miR-143-5p的表达水平则随着CoCl2浓度的升高而下降。结论:CoCl2诱导的化学缺氧对人牙髓干细胞(hDPSCs)促血管生成潜能具有促进的作用,并且该作用呈浓度依赖性。更多还原

【Abstract】 Objectives:To investigate the effects of chemical hypoxia induced by different concentrations of cobalt chloride（CoCl₂） on the angiogenic potential of human dental pulp stem cells（hDPSCs）.Methods:hDPSCs were isolated and cultured in vitro.After surface markers were detected and identified,CoCl₂ with different concentrations（0.05 mM,0.1 mM,0.2 mM,0.5 mM,1.0 mM） was used to construct cell hypoxia model in vitro.The effects of hypoxia on the proliferation and migration of hDPSCs were detected by CCK-8 and cell migration experiments.The mRNA expression levels of has-miR-143-5 p,HIF-1α and VEGF were detected by RTPCR.Results:After 48 h treatment,0.05 mM and 0.1 MM CoCl₂ promoted the proliferation of hDPSCs,while 0.5 mM and 1.0 mM CoCl₂ inhibited the proliferation compared with the control group.CoCl₂ at 0.05 mM,0.1 mM,0.2 mM and 0.5 mM could promote the migration of hDPSCs.The mRNA expression levels of HIF-la and VEGF increased with the increase of CoCl₂ concentration,while the expression levels of has-miR-143-5 p decreased with the increase of CoCl₂ concentration.Conclusion:CoCl₂-induced chemical hypoxia promoted the angiogenic potential of human dental pulp stem cells（hDPSCs） in a concentration-dependent manner.更多还原