【作者】 姚露露； 袁思； 吴震南； 骆建宇； 汤小荣； 唐纯志； 崔帅； 许能贵；

【Author】 Lulu Yao;Si Yuan;Zhennan Wu;Jianyu Luo;Xiaorong Tang;Chunzhi Tang;Shuai Cui;Nenggui Xu;South China Research Center for Acupuncture and Moxibustion,Medical College of Acupuncture Moxibustion and Rehabilitation,Guangzhou University of Chinese Medicine;Research Institute of Acupuncture and Meridian,Anhui University of Chinese Medicine;

【机构】 广州中医药大学针灸康复临床医学院华南针灸研究中心； 安徽中医药大学针灸经络研究所；

【摘要】 目的:观察电针调节对侧感觉皮质(S1)对局灶性单侧运动皮质(M1)梗死的影响。方法:将小鼠随机分成中风组、中风+电针组和伪手术组,每组50只。中风组给予光栓法进行单侧M1局部缺血性梗死的模型复制,激光参数为:波长530 nm,功率15m W,照射10分钟后,激光散斑进行模型评估;中风+电针组对百会、大椎穴进行电针刺激,电针参数:以疏波2 Hz,密波10 Hz,电流1 mA,刺激15 min,连续治疗3天;伪手术组只进行手术操作,不进行激光照射。采用激光散斑评估M1与S1的血流灌注变化,在体电生理双核团记录对侧M1与S1神经活动变化,利用离体膜片钳电生理记录技术检测对侧S1和M1的功能连通性。结果:单侧M1梗死后,对侧M1和S1之间的血流灌注和神经元相互作用受到损害,伪手术组与中风组比较,M1的血流灌注量和神经元放电显著下降(p <0.05);S1的血流灌注量和神经元放电也显著下降(p <0.01)。内源性神经元兴奋性和活动也受到干扰(p <0.05)。通过电针治疗后,对侧M1与S1功能得到了恢复,M1与S1的血流灌注量明显升高(p <0.01);M1与S1的放电频率也得到了提升(p <0.01和p <0.05)。此外,在病毒介导的对侧S1神经元消融后,电针治疗的有效性受到抑制。结论:对侧S1区的神经元活动对于电针介导的局灶性M1梗死后的恢复很重要。揭示了S1-M1环路可能参与电针治疗单侧脑梗死的机制。更多还原

【Abstract】 Objective To observe the effect of electroacupuncture regulation of contralateral sensory cortex(S1) on focal unilateral motor cortex(M1) infarction.Methods Mice were allocated randomly to the following three groups with 50 mice in each group using the random number table method:stroke,stroke + EA,and sham.The model of unilateral M1 local ischemic infarction was replicated by light suppository in stroke group.The laser parameters:wave length 530 nm,power 15mW.After 10 minutes of irradiation,the laser speckle is used to evaluate the model.Stroke + EA group was given EA stimulation at GV20 and GV14.Electroacupuncture parameters:sparse wave 2 Hz,dense wave 10 Hz,current 1 mA,stimulation 15 min,continuous treatment for 3 days.The sham group,only the operation and no laser irradiation was performed.The changes of blood perfusion of M1 and S1 were evaluated by laser speckle,the changes of nerve activity of contralateral M1 and S1 were recorded in vivo,and the functional connectivity of contralateral S1 and M1 was detected by in vitro patch clamp electrophysiological recording technique.Results Blood perfusion and neuronal interaction between contralateral M1 and S1 is impaired after unilateral M1 infarction.Compared with the stroke group,the blood perfusion and neuronal discharge of M1 in the sham group were significantly lower than the stroke group(p <0.05).The blood perfusion and neuronal discharge of S1 also decreased significantly(p < 0.01).Intrinsic neuronal excitability and activity were also disturbed(p < 0.05).After EA treatment,the function of contralateral M1 and S1 was restored,and the blood perfusion of M1 and S1 increased significantly(p < 0.01).The discharge frequencies of M1 and S1 were also increased(p < 0.01 and p < 0.05).Furthermore,the effectiveness of electroacupuncture treatment was inhibited after virusmediated neuronal ablation of the contralateral S1.Conclusion Neuronal activity of the contralateral S1 is important for EA-mediated recovery after focal M1 infarction.Our study provides insight into how the S1–M1 circuit might be involved in the mechanism of EA treatment of unilateral cerebral infarction.更多还原