【作者】 郑明静； 郑雅君； 张一非； 朱艳冰； 杨远帆； 姜泽东； 倪辉；

【Author】 Zheng Mingjing;Zheng Yajun;Zhang Yifei;Zhu Yanbing;Yang Yuanfan;Jiang Zedong;Ni Hui;College of Food and Biological Engineering,Jimei University;Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering;Key Techniques of Deep machining of Marine Food Joint Construction of Provincial and Ministerial Collaborative Innovation Center,Dalian University of Technology;Research Center of Food Biotechnology of Xiamen City;Aquatic Products Processing Technology National Joint Local Engineering Research Center;

【机构】 集美大学海洋食品与生物工程学院； 福建省食品微生物与酶工程重点实验室； 海洋食品精深加工关键技术省部共建协同创新中心大连工业大学； 厦门市食品生物工程技术研究中心； 水产品深加工技术国家地方联合工程研究中心；

【摘要】 红毛藻(Bangia fusco-purpurea)又称为红毛菜,是我国东南沿海特色的食用海藻资源。研究发现红毛藻多糖(BFP)具有免疫调节、抑菌、降血糖等生物活性,在食品和生物医药领域有重要的研究和开发应用价值。本研究针对BFP对人体肠道菌群是否有积极的影响这一问题,采用人源肠道菌群体外三相发酵模型,基于16S rDNA高通量测序和非靶向代谢组学技术研究BFP与肠道菌群相互作用,旨在阐明BFP对肠道菌群结构和功能的调控作用及其潜在的益生功效。结果显示:与对照组相比1%BFP作为碳源发酵提高了微生物的多样性和均匀度,显著增加拟杆菌门和厚壁菌门细菌数量并减少变形菌门细菌数量(P<0.05)。代谢组学结果显示,BFP明显上调瓜氨酸、花生四烯酸、叶酸、烟酸、(R)-3-羟基丁酸、D-半乳糖等差异代谢物。综上,研究结果提示BFP对调节肠道菌群结构与功能具有积极的作用,并通过参与维生素、氨基酸和脂质等代谢途径促进酵解产物中氨基酸、维生素和短链脂肪酸的增加,具有维持肠道上皮屏障、抗炎和抗肥胖等潜在功效。更多还原

【Abstract】 Bangia fusco-purpurea is a characteristic edible seaweed resource in the southeast coast of China.Studies have found that Bangia fusco-purpurea polysaccharide(BFP) has immunomodulatory,antibacterial,hypoglycemic and other activities.It has important research and development application value in the field of food and biomedicine.In this study,we investigated the interaction between BFP and intestinal flora based on 16 S rDNA High-throughput sequencing and Non-targeted metabolomics techniques using an in vitro three-segment fermentation model of human intestinal flora to elucidate the regulatory effect of BFP on the structure and function of gut flora and its potential probiotic efficacy.The results showed that compared with the control group,1 % BFP as carbon source fermentation increased the diversity and uniformity of microorganisms,significantly increased the number of Bacteroidetes and Firmicutes and decreased the number of Proteobacteria(P <0.05).Metabolomics results showed that BFP significantly up-regulated differential metabolites such as citrulline,arachidonic acid,folic acid,niacin,(R)-3-hydroxybutyric acid,and D-galactose.In summary,the results suggest that BFP has a positive effect on regulating the structure and function of intestinal bacteria,and promotes the increase of amino acids,vitamins and short-chain fatty acids in glycolytic products by participating in metabolic pathways such as vitamins,amino acids and lipids,with potential effects such as maintaining the intestinal epithelial barrier,and anti-obesity.更多还原