【作者】 丁香君； 李海丽； 余宇晖； 邵奔； 李梅青；

【Author】 Ding Xiangjun;Li Haili;Yu Yuhui;Shao Ben;Li Meiqing;College of Tea and Food Science and Technology,Anhui Agricultural University;Anhui Engineering Laboratory for Agro-products Processing,Anhui Agricultural University;

【机构】 安徽农业大学茶与食品科技学院； 安徽省农产品加工工程实验室；

【摘要】 本研究旨在探究绿豆肽螯合亚铁（MBP-Fe）的体内补铁功效。对绿豆蛋白进行酶解后,经超滤、Sephadex G-15纯化分离出小分子绿豆肽,采用Nano RP-HPLC与Q Exactive Plus质谱联合鉴定其氨基酸序列,经UniProtKB蛋白库筛选比对得到6条平均分子量为825 u的肽,其中每个肽段都含有一个或多个酸性氨基酸,如Glu和Asp,以及碱性氨基酸,如Arg和Lys。通过动物实验确定MBP与Fe²⁺改善缺铁性贫血（IDA）的体内功效。ICR小鼠随机分为7组:1组为正常对照组,其余6组利用缺铁饲料建立IDA模型后设为1组缺铁模型组和5组缺铁组,缺铁组分别补充甘氨酸亚铁（3.5 mg Fe/kg BW）、MBP协同吸收Fe²⁺（MBP:FeSO₄=8:1,3.5 mg Fe/kg BW）,以及低、中、高剂量MBP-Fe（1.16,1.75,3.5 mg Fe/kg BW）,灌胃28 d。结果发现高剂量MBP-Fe和MBP协同吸收Fe²⁺组（3.5 mg Fe/kg BW）小鼠血清铁、血红蛋白及体重增幅显著增加（P <0.05）,并恢复到正常水平,脾脏系数显著降低,相比于甘氨酸亚铁组。综上,MPH-Fe及MPH协同Fe²⁺吸收在达到有效量后对IDA均具有显著的改善作用,而MPH-Fe效果更优。更多还原

【Abstract】 The purpose of this study was to explore the iron supplementation effect of iron chelating peptide from mung bean（MBP-Fe） in vivo.After enzymatic hydrolysis of mung bean protein,the small molecule mung bean peptides were separated by ultrafiltration and Sephadex G-15 purification.The amino acid sequences were identified by Nano RP-HPLC and Q Exactive Plus mass spectrometry.Six peptides with an average molecular weight of 825 u were obtained by screening and comparison of the UniProtKB protein library.Each peptide contained one or more acidic amino acids,such as Glu and Asp,and basic amino acids,such as Arg and Lys.In-vivo efficacy of MBP and Fe²⁺ in improving iron deficiency anemia（IDA） was determined through animal studies.ICR mice were randomly divided into 7 groups:Group 1 was the normal control group,and the other 6 groups were set up by IDA model of iron deficiency diet,which were divided into 1 iron deficiency model group and5 iron deficiency groups.The iron deficiency groups were supplemented with iron glycine（3.5 mg Fe/kg BW） and MBP co-absorption of Fe²⁺（MBP:FeSO₄=8:1,3.5 mg Fe/kg BW）,and low,medium and high doses of MBP-Fe（1.16,1.75,3.5 mg Fe/kg BW） respectively once per day via intragastric administration for 28 days.The results showed that the serum iron,hemoglobin and body weight of mice in the high dose MBP-Fe and MBP co-absorption Fe²⁺ group（3.5 mg Fe/kg BW） were significantly increased（P <0.05）,and returned to the normal level,and the spleen coefficient was significantly decreased,compared with the ferrous glycine group.In conclusion,both MPH-Fe and MPH co-absorption of Fe²⁺ have significant improvement on IDA after reaching the effective amount,and MPH-Fe has the better effect.更多还原