【作者】 刘庆梅； 王亚婷； 康馨文； 陶红群； 党媛；

【Author】 Liu Qingmei;Wang Yating;Kang Xinwen;Tao Hongqun;Dang Yuan;Chengdu Academy of Environmental Protection Sciences;

【机构】 成都市环境保护科学研究院；

【摘要】 通过对比矿化垃圾强化微生物修复5组不同浓度石油污染土壤的总石油烃(TPH)去除率、降解动力学、酶活性、微生物量、微生物多样性和群落组成,分析了土壤初始石油浓度对矿化垃圾强化微生物修复石油污染土壤的效果和微生物学特征的影响。结果表明:土壤石油含量为5%(w/w)时,矿化垃圾对TPH的去除效果最好,去除率为89.83%;TPH的生物降解速率最高,半衰期最短(13.59 d)。5组试验TPH去除率顺序为5%石油(89.83%)> 3%石油(87.79%)>10%石油(66.73%)>20%石油(38.76%)>30%石油(26.98%)。石油含量≤5%时,石油污染物对土壤微生物的毒性较低,增加一定浓度的石油为微生物提供了更多碳源和能源,提高了微生物数量和活性,进而提高了TPH去除率;石油含量>5%时,石油污染物表现出对土壤微生物的抑制作用,微生物总活性随着石油浓度的增加而降低,去除率也显著下降。3%石油试验组与其他试验组微生物群落结构之间的差异随着石油浓度的增加而变大。5组试验中优势细菌属和真菌属分别为诺卡氏菌属和柄孢壳属,诺卡氏菌属的占比随着石油浓度的增加而增大,而柄孢壳属的占比随着石油浓度的增加而减小。更多还原

【Abstract】 The effects of initial petroleum content in soil on the enhanced bioremediation of petroleum-contaminated soil using aged refuse were investigated by comparing total petroleum hydrocarbon(TPH) removal rate,degradation kinetics,enzyme activities,microbial biomass,microbial diversity and community composition in five treatments with different petroleum content.The results demonstrated that 5% petroleum treatments showed the highest TPH removal rate(89.83%),the highest TPH biodegradation rate and the shortest half-life(13.59 d),and the TPH removal rate in soil of these treatments was ranked in the order:5% TPH(89.83%) > 3% TPH(87.79%) > 10% TPH(66.73%) > 20% TPH(38.76%) > 30% TPH(26.98%).When the TPH content was ≤ 5%,the toxicity of petroleum pollutants was low.TPH content increase could result in more abundant carbon sources and energy for soil microorganisms,which could improve the quantity and activity of microorganisms,and thus improve the TPH removal rate.When the TPH content was > 5%,TPH removal rate decreased with the increase of TPH concentration due to the toxic effect of petroleum pollutants and low total microbial activity.The difference of microbial community structure between 3% TPH level treatment and other treatments increased with the increase of the TPH concentration.The dominant bacteria and fungi in the soils of five treatments were all Nocardia and Zopfiella,respectively.The proportion of Nocardia to total bacteria increased with the increase of the TPH concentration,while the proportion of Zopfiella was opposite.更多还原