【作者】 龚毅豪； 刘淇； 赵红玲； 孔福全； 王巧娟； 黎雨尘； 章雯； 周平坤； 关华； 隋丽；

【Author】 GONG Yihao;LIU Qi;ZHAO Hongling;KONG Fuquan;WANG Qiaojuan;LI Yuchen;ZHANG Wen;ZHOU Pingkun;GUAN Hua;SUI Li;Department of Nuclear Physics, China Institute of Atomic Energy;National Innovation Center of Radiation Application;Department of Radiation Toxicology and Oncology,Beijing Key Laboratory for Radiobiology, Beijing Institute of RadiationMedicine;

【机构】 中国原子能科学研究院核物理研究所； 国防科技工业抗辐照应用技术创新中心； 军事科学院军事医学研究院辐射医学研究所放射生物学北京市重点实验室；

【摘要】 目的研究VND3207对14MeV单能中子导致的Wistar大鼠造血和免疫系统损伤的防护作用。方法采用称量方法检测了大鼠体重和脏器指数的变化,全血细胞计数方法检测了血细胞的数量变化及形态分布情况、流式细胞仪检测了血细胞表面的T淋巴细胞亚群,病理切片方法检测了大鼠脏器组织的病理改变。结果与照射组相比,药物干预照射组大鼠的体重和脏器指数增加;血细胞生化指标明显恢复,有核细胞占全细胞比例数提高,粒红比降低;胸腺和脾脏病理损伤有所减轻,肝脏出现kupffer细胞(吞噬细胞)及分叶核细胞的少量浸润;CD8+T细胞所占比例明显增加,CD4+T细胞与CD8+T细胞比值恢复至2比1的正常水平。结论 VND3207具有减少中子辐射致造血功能损伤及促进其恢复的功效,可减轻中子辐射导致的抑制性T淋巴细胞损伤,降低大鼠机体对中子辐射的敏感性,提高大鼠机体的免疫功能。因此,VND3207对中子诱发大鼠辐射损伤有良好的保护作用,有开发成为抗高LET辐射损伤新药的价值。更多还原

【Abstract】 Objective To study the radioprotection of VND3207 in hematopoiesis and the immune system of Wistar rats induced by 14 MeV neutrons. Methods The body weight and viscera index of rats were weighted, the number and morphological distribution of blood cells were measured by the complete blood count(CBC), the T lymphocyte subsets on the surface of blood cells was analyzed by flow cytometry, the tissues of rat were examined by the pathological section. Results For the irradiation rats with VND3207 treatment, compared with the irradiation rats, the body weight and organ index increased; the biochemical indexes of blood cells obviously recovered, the proportion of nucleated cells in the whole cells increased, and the ratio of granulocytes to erythrocytes decreased; the damage of thymus and spleen was released, and the liver appeared small infiltration between Kupffer cells(phagocytes) and lobulated nuclear cells; the proportion of CD8+T cells increased significantly, and the ratio of CD4+T cells to CD8+T cells recovered to the normal level(2:1). Conclusions VND3207 has the effect of reducing hematopoietic damage and promoting its recovery caused by neutron radiation. VND3207 can reduce the inhibitory T lymphocytes damage and the sensitivity of rats to neutron, and improve the immune function of the rats. VND3207 may have a certain therapeutic effect for neutron radiation damage.更多还原