【作者】 冯佳萌； 黄梦影； 郝琳； 郭琳娜； 王钎钎； 高书涛； 商宁昭； 王春； 王志；

【Author】 Mengjia Feng;Yingmeng Huang;Lin Hao;Linna Guo;Qianqian Wang;Shutao Gao;Ningzhao Shang;Chun Wang;Zhi Wang;College of Science,Hebei Agricultural University;

【机构】 河北农业大学理学院；

【摘要】 左氧氟沙星是一种广谱口服氟喹诺酮类抗菌剂,广泛应用于临床系统感染的治疗^[1,2]。目前,抗生素滥用已成为威胁全球公共卫生的严重社会问题^[3],因此开发一种廉价、简单、快速、准确定量检测左氧氟沙星的方法具有重要意义。本文采用直接热解三嗪共价微孔聚合物（MCTPs）的方法,制备了包埋Al₂O₃的纳米碳球,用于左氧氟沙星电化学传感器的构建。Al₂O₃-C₈₀₀微球具有较大的比表面积,出色的导电性,因此大大提高了左氧氟沙星在电极上的氧化电流。结果显示所制备的Al₂O₃-C₈₀₀修饰的传感器用于左氧氟沙星的检测具有较低的检出限(0.5μmol L^-1)和较宽的线性范围(2.0-50.0μmol L^-1),可成功用于人血清中左氧氟沙星的选择性测定。更多还原

【Abstract】 Levofloxacin,a broad spectrum oral fluoroquinolone antibacterial agent,can be used for the treatment of a variety of infections^[1,2].Now antibiotic abuse has become a serious social issue threatening public health all over the world^[3].Therefore,the development of inexpensive,simple,rapid and accurate quantitative detection method for levofloxacin is of highly essential.A highly sensitive electrochemical sensor for the determination of levofloxacin was developed based on carbon microsphere embedded Al₂O₃（Al₂O₃-C） modified glassy carbon electrode.Al₂O₃-C microsphere was fabricated by direct pyrolysis of microporous covalent triazine polymer.The Al₂O₃-C microsphere exhibits large surface area,excellent conductivity and greatly boosts the oxidation current of levofloxacin on the electrode.Under the optimal conditions,a good linearity relationship was observed between the current of oxidation peak and the concentration of levofloxacin in the range of 2.0-50.0 μmol L^-1 with a detection limit（S/N=3） of 0.5 μmol L^-1.The sensor based Al₂O₃-C can be feasibly employed for the determination of levofloxacin in human blood serum samples with excellent selectivity,high sensitivity,and good reproducibility.更多还原