【作者】 谈蓉； 沈一； 李冬宁； 杨娅； 屠一锋；

【Author】 Rong Tan;Yi Shen;Dongning Li;Ya Yang;Yifeng Tu;College of Chemistry,Chemical Engineering and Materials,Soochow University;Department of Forensic Medicine,Institute of Forensic Science,Soochow University;Dushu Lake Campus of Soochow University;

【机构】 苏州大学材料与化学化工学部； 苏州大学司法鉴定中心；

【摘要】 甲基苯丙胺(METH)俗称冰毒,是一种强效的中枢神经系统兴奋剂,令人产生快感的同时,也可导致焦虑、精神病性障碍、失眠及多动等一系列精神后遗效应,严重危害人类身心健康。因制造简单,成本低廉,利润丰厚,METH成为日渐风靡的滥用物质,各国政府已纷纷加强监管。不过要从生物样本中检测冰毒,往往需要特定的场所、昂贵的仪器以及专业的操作人员,且耗时较长。因此,开发一种准确、灵敏、快速、方便的METH检测技术亟不可待。本文结合免疫亲和的高选择性与电化学发光(ECL)的高灵敏度提出了两个冰毒传感器。AuNPs功能化的聚乙烯对苯二甲酸酯(PET)(含氧化铟锡(ITO)导电涂层)为基础电极,固定化的免疫元件实现靶标的特异性识别;配备性能优异的便携式发光仪,从而实现执法现场的快速检测。通过实验验证,两个传感器均达到METH的高灵敏检测;实样鉴定也进一步证明了这种方法的实用性和准确性。本项工作意义重大,在药物滥用的现场快速检测方面具有指导作用。更多还原

【Abstract】 The abuse of methamphetamine(METH) has an obvious increase during past decades,seriously jeopardized the physical and mental health of human.It compelled growing worry worldwide so that there is a strong requirement to develop the accurate,sensitive,rapid,and convenient detection techniques to tackle the problem.In regard to this matter,we reported two sensors for METH or its antibody with synergistical combination of the sensitivity of electrochemiluminescent(ECL) and the selectivity of immune affinity.These sensors are applicable for point-of-care testing when equipped a portable ECL meter with outstanding performance,makes on-spot analysis come true.The dual-way analysis of immunosensors can be carried on,both exhibit unprecedented sensitivity toward METH and its antibody.Here,AuNPs functioned indium tin oxide(ITO) coated polyethylene terephthalate(PET) is used as the basal electrode,providing excellent ECL background and supporting the sensing matrix.The immobilized immune elements play the role of escort for specific target recognition.Real sample identification further demonstrated the practicability and accuracy which is a vital problem in forensic identification and clinical treatment.This work provides a viable methodology and technology for a rapid,reliable,and on-spot analysis of drug abuse.更多还原