【作者】 肖克； 付亚敏； 张小华； 杜翠翠； 陈金华；

【Author】 Ke Xiao;Yamin Fu;Xiaohua Zhang;Cuicui Du;Jinhua Chen;State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University;

【机构】 湖南大学化学生物传感与计量学国家重点实验室；

【摘要】 基于集识别与放大信号为一体的ZrO₂/CdS八面体,我们构建了一种免标记、近零背景的光电化学生物传感器实现了蛋白激酶（PKA）活性的灵敏检测,并将其应用于PKA抑制剂的筛选。以Zr-MOF（UiO-66）为前驱体成功合成了尺寸均匀,比表面积大的ZrO₂八面体。采用CdS与ZrO₂八面体复合后,其光电转换效率显著提升。ZrO₂/CdS不仅可以与PKA催化多肽产生的磷酸基团特异性结合,而且可以作为信号探针产生光电信号。该生物传感器对PKA的检测呈现出高灵敏度,检出限约为0.00035 U mL^-1 （S/N=3）,且在与激酶有关的生命科学和药物领域显示出潜在的应用前景。更多还原

【Abstract】 Based on the ZrO₂/CdS octahedra that integrate recognition and signal amplification,we constructed a label-free,near-zero background photoelectrochemical biosensor to detect the activity of protein kinase（PKA） and applied it to inhibitors of screening.The porous ZrO₂ octahedra with uniform size and large surface area were successfully synthesized by taking Zr-MOF（UiO-66） as the precursor.After the modification of CdS,the photoelectric conversion efficiency of ZrO₂ octahedra is significantly improved.The prepared CdS/ZrO₂ octahedron can not only specifically interact with phosphorylated peptide,but also can act as signal probe to produce sharp photocurrent.The developed sensor shows high sensitivity for the detection of PKA,with a detection limit of about 0.00035 U mL^-1（S/N=3）,and shows potential application prospects in the life sciences and pharmaceutical fields related to kinases.更多还原