【作者】 杨志刚； 屈军乐；

【Author】 Zhigang Yang;Junle Qu;Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Optoelectronic Engineering,Shenzhen University;

【机构】 深圳大学光电子器件与系统教育部/广东省重点实验室深圳大学光电工程学院；

【摘要】 本文设计了一类可用于选择性标记活细胞线粒体膜邻位二巯基蛋白质的荧光探针,可对其进行共价标记。探针可以进行活细胞的超分辨成像研究,在低功率下实现高分辨率的活细胞线粒体膜结构成像研究,以及线粒体的动态变化过程监测。有望为超分辨成像研究提供全新的荧光探针及为线粒体的超精细结构成像提供新的手段。更多还原

【Abstract】 A series of new fluorescent sensors were developed to target for the vicinal dithiol proteins on the mitochondrial membrane in live cells. And the probes can carry out the superresolution imaging of mitochondrial membrane structures in live cells with low STED laser power. Moreover, it is able to monitor the dynamic changes of mitochondria with a lot of details, which was proved to be a promising fluorescent probe for superresolution imaging in live cells and to provide powerful tools for the investigation of ultra-fine structures of mitochondria.更多还原