【作者】 胡雪； 陈晴； 张丹丹； 陈旭伟； 王建华；

【Author】 Xue Hu;Qing Chen;Dan-DanZhang;Xuwei Chen;Jianhua Wang;Research Center for Analytical Sciences, Department of Chemistry, College of Sciences,Northeastern University;

【机构】 东北大学理学院化学系分析科学研究中心；

【摘要】 采用溶剂热法将吡啶硼酸固载到PW₁₂@Ti O₂-Si（Et）Si上制备了多孔杂化材料PW₁₂@Ti O₂-Si（Et）Si/Pba。硼酸基团与蛋白质的糖基化位点间的相互作用使PW₁₂@Ti O₂-Si（Et）Si/Pba杂化材料对糖蛋白表现出良好的的吸附性能。在p H9.0时,该杂化材料对Ig G和Trf的吸附容量为348.5mg g^-1和262.6mg g^-1。将该杂化材料应用于人血清中高丰度糖蛋白的吸附去除,Trf、触珠蛋白、IgG和IgM的百分比分别从7.38%,3.01%,5.5%和1.02%降至0.951%,0.126%,4.7%和0.76%。与原始样品相比,去除高丰度糖蛋白后的血清样品中可鉴定85种新低丰度蛋白,例如类风湿因子、蛋白质IGKV3-11、抗H1N1流感HA和c DNA FLJ53691等,表明了所制备的PW₁₂@Ti O₂-Si（Et）Si/Pba杂化材料在蛋白质组学样品预处理方面具有应用潜力。更多还原

【Abstract】 The porous hybrid PW₁₂@Ti O₂-Si（Et）Si/Pba is obtained by loading boronic acid onto PW₁₂@Ti O₂-Si（Et）Si via a solvothermal method.The interactions between boronic acid group and the glycosylation site of proteins offer PW₁₂@Ti O₂-Si（Et）Si/Pba favorable adsorption performance towards glycoproteins.The adsorption capacities for IgG and Trf are 348.5 mg g^-1 and 262.6 mg g^-1 under pH 9.0.The obtained hybrid is applied to deplete high abundant glycoproteins from human serum.Compared with original plasma sample,the percentages of Trf,haptoglobinis,IgG and IgM are reduced from 7.38%,3.01%,5.5%and 1.02%to 0.951%,0.126%,4.7%and 0.76%.Meanwhile,85 new low-abundance proteins such as rheumatoid factors,protein IGKV3-11,anti-H1N1 influenza HA and c DNA FLJ53691 can be identified.These results well demonstrate the great potential of PW₁₂@Ti O₂-Si（Et）Si/Pba in the sample pretreatment of proteomics.更多还原