【作者】 刘玉申； 赵超； 王娟； 李娟；

【Author】 Yushen Liu;Chao Zhao;Juan Wang;Juan Li;School of Public Health, Jilin University;

【机构】 吉林大学公共卫生学院；

【摘要】 副溶血性弧菌(V. parahaemolyticus)是最常见的食源性病原体之一。人们食用受该菌污染的食品后,极易引起副溶血弧菌食物中毒。本文描述了基于免疫磁珠的高效富集和纳米模拟酶的信号放大作用对副溶血性弧菌的快速比色测定法。使用Mn O2纳米颗粒作为人工氧化酶,在氧气存在下将3,3’,5,5’-四甲基联苯胺氧化形成652nm处有最大吸收峰的蓝色产物;将免疫磁珠的优异捕获效率与纳米模拟酶的高催化活性相结合,在不进行预富集的情况下,副溶血弧菌的检测浓度为10至105 cfu·mL-1,检测限低至10cfu·mL-1,且不受其他杂菌的干扰。分析加标牡蛎样品时发现回收率在87.5%-106.0%。本实验建立的检测方法具有快速,灵敏且特异性高的特点,有希望应用于副溶血性弧菌的快速和现场视觉检测。更多还原

【Abstract】 Vibrio parahaemolyticus（V. parahaemolyticus） is one of the most common food-borne pathogens.Food poisoning can be easily caused by people eating food contaminated with V. parahaemolyticus. The authors describe a rapid colorimetric assay for V. parahaemolyticus that is based on a combination of a magnetic bead-based sandwich immunoassay and signal amplification via an enzmye mimic. MnO2 nanoparticles are used as an artificial oxidase that oxidizes 3,3′,5,5′-tetramethylbenzidine in the presence of oxygen to form a blue product. By combining the superior capture efficiency of magnetic beads with the high catalytic activity of the enzmye mimic, this method can detect V. parahaemolyticus concentration between 10 to 105 cfu·mL^-1 without pre-enrichment, and the limit of detection is 10 cfu·mL^-1. Recoveries ranging from 87.5% to 106.0% are found when analyzing spiked oyster samples. The assay is rapid, sensitive, and specific. In our perception, it shows promise in rapid and on-site visual detection of V. parahaemolyticus.更多还原