【作者】 唐晓娣； 雷小玲； 吴小华； 李蕾； 尹争志；

【Author】 Xiaodi Tang;Xiaoling Lei;Xiaohua Wu;Lei Li;Zheng-Zhi Yin;College of Chemistry and Chemical Engineering, Zhejiang Normal University;College of Biological and Chemical Engineering, Jiaxing University;

【机构】 浙江师范大学化学与生命科学学院； 嘉兴学院生物与化学工程学院；

【摘要】 以微囊藻毒素（MC-LR）为检测研究目标,构筑其紫外可见免疫传感体系^[1,2]。MC-LR的适配体和抗体分别功能化PDA@CuNPs（铜纳米粒子包覆聚多巴胺球）和Fe₃O₄@SiO₂（二氧化硅包覆的磁性纳米粒子）,进而双位点特异性识别结合MC-LR的相应靶点,形成三明治夹层型复合物;磁性快速分离并将复合体中的铜转化为离子(Cu²⁺),进一步与双环己酮草酰二腙（BOC）反应,形成turn-on型紫外可见吸收响应。通过TEM、SEM、TGA和FTIR等对Fe₃O₄@SiO₂、PDA@CuNPs复合物进行表征。将实验结果与近十年的MC-LR文献指标相比较,该传感器在检测范围和检测限等方面,都得到了一定的改善,表现出较高的特异性。该检测体系方法简单、灵敏度高、选择性好,实际样品检测性优良。为检测MC-LR提供了潜在的有效方法,具有一定的借鉴意义。更多还原

【Abstract】 Microcystins （MC-LR） was selected as a detection target to construct its UV-vis immunosensor.The PDA@CuNPs and Fe₃O₄@SiO₂ were functionalized with the aptamer and antibody of MC-LR respectively,the specific two-site recognition combined the corresponding target of MC-LR and forming a sandwich complex.After rapidly magnetic separation,the copper in the complex was converted into ions (Cu²⁺),and further reacted with bicyclohexanone oxaloyl dioxime （BOC） to form a turn-on UV-vis signal.The Fe₃O₄@SiO₂ and PDA@CuNPs complexes were characterized by TEM,SEM,TGA and FTIR.Comparing the experimental results with the MC-LR literature of the past decade,the sensor has been improved in terms of detection range and detection limit,showing a high specificity.The detection system has the advantages of simple method,high sensitivity,good selectivity and sample detection performance.For the detection of MC-LR,it provided a potentially effective method.更多还原