【作者】 薛诗凡； 陈子晗； 韩欣悦； 施国跃； 张闽；

【Author】 Shi-Fan Xue;Zi-Han Chen;Xin-Yue Han;Guoyue Shi;Min Zhang;School of Chemistry and Molecular Engineering, East China Normal University;

【机构】 华东师范大学化学与分子工程学院；

【摘要】 在本工作中,我们筛选了一系列富含鸟嘌呤/胸腺嘧啶（G/T）的DNA配体,以敏化Tb³⁺发光作为智能传感元件,响应溶液中的各种金属离子。通过不同金属离子与DNA的相互作用,使得由DNA朝向Tb的能量转移出现不同程度的差异。我们的研究结果表明,仅需3段无标记的DNA与Tb构成的传感元件,可成功区分49种金属离子,涵盖了碱金属,碱土金属,过渡/过渡后金属和镧系元素。49种金属的盲测结果进一步证实了DNA/Tb传感元件的区分能力。此外,我们拓展了基于DNA/Tb传感元件荧光寿命对不同金属离子的模式识别分析结果。更多还原

【Abstract】 In this work, a series of guanine/thymine（G/T）-rich DNA ligands was screened to sensitize the luminescence of Tb³⁺ as smart pattern responders to metal ions in solution, and metal ion-DNA interactions can differentially alter the energy transfer of DNA toward Tb as pattern signals. Our results show that only 3 DNA/Tb label-free sensors could successfully discriminate 49 metal ions, including alkali-metal, alkaline-earth-metal, transition/post-transition metal, and lanthanide. A blind test with 49 metals further confirmed the discriminating power of DNA/Tb sensors. Moreover, the lifetime-based pattern recognition analysis using DNA/Tb sensors was also developed.更多还原