【作者】 陈卓； 张鲁凤； 张良； 董倩； 刘张坤；

【Author】 Zhuo Chen;Lufeng Zhang;Liang Zhang;Qian Dong;Zhangkun Liu;College of Chemistry and Chemical Engineering, Hunan University;

【机构】 湖南大学化学化工学院；

【摘要】 拉曼光谱技术作为一种振动光谱技术,已成为分子医学研究领域中有价值的检测手段。围绕拉曼技术在生物医学检测与成像中的问题,我们针对性地开展功能碳纳米材料设计,进行了较系统的研究。通过将贵金属纳米粒子包裹在石墨烯薄层中,我们设计构造了贵金属石墨纳米囊拉曼生化分析平台。我们研制了超级稳定的银纳米颗粒,并通过功能化修饰核酸适配体等分子,实现了对肿瘤细胞的靶向、低背景、快速拉曼成像。我们还研制了金石墨纳米囊复合纳米结构,结合拉曼与双光子荧光成像等模式,实现了对细胞的多模态检测与成像,进一步提高了成像的灵敏度。我们还设计了金纳米粒子修饰的磁性多层石墨纳米囊体系,并利用其作为内标和增强基底消除了常规传统内标分子的不稳定有背景干扰等问题,提高了拉曼检测的准确性。基于石墨纳米囊的拉曼分析平台的建立,为其在生物医学等复杂体系的应用提供有效的工具显示了广阔的前景。更多还原

【Abstract】 Raman spectroscopy offers certain fingerprints to identify chemical compounds. Graphitic nanocapsules are emerging nanomaterials which are gaining popularity along with the development of carbon nanomaterials. Graphitic nanocapsule has demonstrated extraordinary performance as both SERS substrate for Raman bioimaging and analysis. We fabricated corrosion-resistant, water-soluble, and graphene-protected AgCu nanoparticles(ACG). Such stable ACG have been utilized for cell labelling, rapid Raman imaging and SERS detection. We also developed a graphene-isolated-Au-nanocrystal(GIAN) nanostructures. Multimodal cell imaging and NIR photothermal-enhanced chemotherapy have been realized with such GIAN platform. Cancer cell-specific aptamers were further functionalized on the graphitic surface through simple modification to realize imaging selectivity through differential cancer cell recognition. All these results indicate graphitic nanocapsule is an optimum choice for SERS analysis in complex systems.更多还原