【作者】 冯涛涛； 张悦； 张美宁；

【Author】 Taotao Feng;Yue Zhang;Meining Zhang;Department of Chemistry, Renmin University of China;

【机构】 中国人民大学化学系；

【摘要】 碳纤维微电极（CFME）被广泛植入大脑组织检测神经传递。植入后的微电极表面会吸附生物分子形成一层膜,从而导致电极灵敏度和选择性变差。因此,我们通过电聚合可控的构建了单宁酸掺杂多孔聚苯胺（TA-pPani）抗蛋白吸附涂层。利用多孔聚苯胺与单宁酸之间的协同效应,电极涂层可以有效抵抗蛋白质非特异性吸附并能够富集神经递质（DA）,提高微电极对体内电化学检测DA的选择性和灵敏度。实验结果表明:活体后的TA-pPani/CFME的灵敏度(S_post)与活体前校准的灵敏度(S_pre)几乎相同。因此,该涂层能够有效地减少蛋白质非特异性吸附,大大提高了体内检测DA的可靠性,有助于理解DA的生理学和病理学功能。更多还原

【Abstract】 Carbon-fiber microelectrode （CFME） is widely used for detection of neurotransmission in vivo but is limited by poor sensitivity caused the biofouling on CFME surface.We demonstrate an TA-pPani coating with the goal of creating a controllable antifouling coating.The coating can be resisting protein adsorption and enriching dopamine to increase the DA sensitivity of implanted electrodes in rat brain.The experiment results show that the sensitity of anti-biofouling electrode of post-calibration (S_post) is same as pre-calibration (S_pre).Thus,TA-pPani coating have high resistance to adsorption of proteins and improved reliability for tracking DA.更多还原