【作者】 余琼卫； 郑凤； 方凯敏； 冯钰锜；

【Author】 YU Qiongwei;ZHENG Feng;FANG Kaimin;FENG Yuqi;Key Laboratory of Analytical Chemistry for Biology and Medicine,Ministry of Education,College of Chemistry and Molecular Sciences,Wuhan University;

【机构】 生物医学分析化学教育部重点实验室武汉大学化学与分子科学学院；

【摘要】 采用液相沉积法(LPD)制备了纳米氧化锆沉积硅胶色谱固定相(ZrO2/SiO2),并将其应用于亲水作用色谱分离中。考察并比较了ZrO2/SiO2、硅胶(Si02)和氧化锆(ZrO2)3种色谱固定相在不同有机调节剂比例、不同pH值及不同盐浓度的流动相条件下的色谱行为。结果表明,制备的ZrO2/SiO2色谱柱不仅具有SiO2色谱柱高柱效的优点,表面沉积的纳米氧化锆还能有效屏蔽硅羟基,有利于碱性物质的保留和分离,表现出良好的亲水作用色谱性能。将ZrO2/SiO2色谱柱用于4种脱氧核苷和5种碱性化合物的分离,均得到了较好的效果,展现出其作为色谱固定相良好的应用前景。更多还原

【Abstract】 ZrO₂ nanoparticles were deposited on silica by LPD method and the resulting ZrO₂/SiO₂ was employed as stationary phase in hydrophilic-interaction liquid chromatography.The chromatographic performance of three columns packed with SiO₂,ZrO₂/SiO₂ and ZrO₂ were compared.The results showed that the column packed with ZrO₂/SiO₂ not only had the advantages of high column efficiency like the SiO₂ column,but also the deposited zirconia nanoparticles on the silica surface could shield the silicon hydroxyl group effec-tively,which benefit for the retention and separation of alkaline substances and showed good hydrophilic-inter-action chromatographic performance.Effective separation of 4 deoxynucleosides and 5 basic compounds was achieved on ZrO₂/SiO₂ column in the hydrophilic-interaction liquid chromatography（HILIC） mode.更多还原