【作者】 聂述琳； 朱锋； 孟翔峰； 聂蓉蓉；

【Author】 Nie Shulin;Zhu Feng;Meng Xiangfeng;Nie Rongrong;Dept.of Prothodontics,The Affiliated Stomatological Hospital, Medical School of Nanjing University,Nanjing Stomatological Hospital;

【机构】 南京大学医学院附属口腔医院·南京市口腔医院；

【摘要】 目的 :通过体外细胞培养模拟牙脱位保存模型,研究不同保存介质和保存温度对人牙周膜细胞活性和增殖能力的影响。方法 :选择三种易于获得的保存介质牛奶、唾液和纯净水,分别在室温和4℃条件下作用于hPDLF 1h,然后利用EdU和CCK-8实验检测作用后的hPDLF细胞活性和后期增殖能力。结果:EdU结果显示,在4℃保存温度下,牛奶处理hPDLF细胞1h后,EdU阳性细胞(绿色)最多,占总细胞的比率约为50.9%。CCK-8结果显示4℃下牛奶、唾液、纯净水中hPDLF活力依次约为62.8%、17.8和3.3%,室温下则依次为56.2%、15.2和0.7%。培养24h、48h、72h后,细胞保存于4℃牛奶中生长曲线最接近于正常对照,与保存于唾液和纯净水中的细胞相比,细胞增殖速率具有显著的统计学差异。结论 :hPDLF在4℃牛奶中表现出了最佳的细胞活力和增殖能力,因此低温牛奶是外伤脱位牙首选的保存液。更多还原

【Abstract】 Objective: The effects of different storage media and temperature on the activity and proliferation of human periodontal ligament fibroblasts cells were studied by simulating the storage model of avulsed tooth in vitro cell culture. Methods: Three readily available storage media, milk, saliva and purified water, were selected and applied to hPDLF for 1 h at room temperature and 4℃, respectively. Then, Ed U and cck-8 were used to detect the viability and proliferation capacity of hPDLF cells after treatment. Results: EdU showed that after milk was treated with hPDLF cells at 4℃ for 1 h, the most cells were edupositive(green), accounting for about 50.9% of the total cells. CCK-8 showed that the hPDLF viability in milk, saliva and purified water at 4℃ was 62.8%, 17.8 and 3.3% respectively, and 56.2%, 15.2 and 0.7% respectively at room temperature. After 24 h, 48 h and 72 h of culture, the growth curve of cells stored in milk at 4℃ was the closest to the normal control. Compared with cells stored in saliva and purified water, the proliferation rate of cells was statistically significant. Conclusions: hPDLF showed the best cell viability and proliferation ability in 4 ℃ milk, so low temperature milk is the preferred preservation solution for avulsed teeth.更多还原