Raman Observing Dynamics and Activation Mechanism of Apoptosis Triggered by Photothermal Therapy

【作者】 邢应芳； 罗晓俊； 康土丽； 祝晶甜； 吴萍； 蔡称心；

【机构】 南京师范大学化学与材料科学学院；

【摘要】 Plasmonic nanoparticle(NP)-mediated photothermal therapy(PPTT) has been explored as a minimally invasive approach to cancer therapy and progressed from concept to early stage of clinic trials.~1 Better understanding the cellular and molecular response to PPTT is crucial for improvement of therapy efficacy and advancement of clinic application.However,the molecular mechanism underlying PPTT-induced apoptosis is still unclear and under dispute.In this work,we used Au nanostars(Au NSs) as both a photothermal agent to specifically induce apoptosis in cancer cells and as a surface enhanced Raman spectroscopy(SERS) probe to monitor the time-dependent SERS spectra of MCF-7 cells which are undergoing apoptosis.Through SERS spectra and their synchronous/asynchronous SERS correlation map,the occurrence and dynamics of a cascade of molecular events have been investigated,and a molecular signaling pathway of PPTT-induced apoptosis,including release of cytochrome c,protein degradation,and DNA fragmentation,was revealed.This work is beneficial for not only improving the fundamental understanding of the molecular mechanism of apoptosis induced by PPTT but also for guiding how to modulate PPTT to drive forward its clinical application.更多还原

【Abstract】 Plasmonic nanoparticle(NP)-mediated photothermal therapy(PPTT) has been explored as a minimally invasive approach to cancer therapy and progressed from concept to early stage of clinic trials.~1 Better understanding the cellular and molecular response to PPTT is crucial for improvement of therapy efficacy and advancement of clinic application.However,the molecular mechanism underlying PPTT-induced apoptosis is still unclear and under dispute.In this work,we used Au nanostars(Au NSs) as both a photothermal agent to specifically induce apoptosis in cancer cells and as a surface enhanced Raman spectroscopy(SERS) probe to monitor the time-dependent SERS spectra of MCF-7 cells which are undergoing apoptosis.Through SERS spectra and their synchronous/asynchronous SERS correlation map,the occurrence and dynamics of a cascade of molecular events have been investigated,and a molecular signaling pathway of PPTT-induced apoptosis,including release of cytochrome c,protein degradation,and DNA fragmentation,was revealed.This work is beneficial for not only improving the fundamental understanding of the molecular mechanism of apoptosis induced by PPTT but also for guiding how to modulate PPTT to drive forward its clinical application.更多还原