Genetic analysis and identification of two soybean mosaic virus-resistance genes in soybean Glycine max(L.) Merr

【作者】 任锐； 智海剑；

【机构】 南京农业大学农学院/国家大豆改良中心/农业部大豆生物学与遗传育种；

【摘要】 Soybean mosaic virus(SMV) commonly affects soybean production worldwide,and the SC18 strain has been widespread in China.The present study aims to characterize and map the SC18-resistance genes present in soybean cultivars Kefeng No.1 and Qihuang 22.Inheritance analysis revealed that two independent single dominant genes in Kefeng No.1 and Qihuang 22 confer resistance to SC18.Using SSR markers and bulked segregant analysis(BSA),the Kefeng No.1 and Qihuang 22 resistance genes were located on soybean chromosomes 2 and 13,respectively.We further screened two populations of recombinant inbred lines(RIL) with 32 simple sequence repeat(SSR) markers in the target region,where the resistance gene in Kefeng No.1 was fine mapped to an 80-kb region containing six putative genes.Sequence and expression analyses of these genes revealed that SMV-resistance in Kefeng No.1 was probably attributable to three of the candidate genes(i.e.,Glyma.02 G127800,Glyma.02 G128200 and Glyma.02 G128300).Collectively,the results of this study will greatly facilitate the cloning of SC18-resistance genes and marker-assisted breeding of SMV-resistant soybean cultivars.更多还原

【Abstract】 Soybean mosaic virus(SMV) commonly affects soybean production worldwide,and the SC18 strain has been widespread in China.The present study aims to characterize and map the SC18-resistance genes present in soybean cultivars Kefeng No.1 and Qihuang 22.Inheritance analysis revealed that two independent single dominant genes in Kefeng No.1 and Qihuang 22 confer resistance to SC18.Using SSR markers and bulked segregant analysis(BSA),the Kefeng No.1 and Qihuang 22 resistance genes were located on soybean chromosomes 2 and 13,respectively.We further screened two populations of recombinant inbred lines(RIL) with 32 simple sequence repeat(SSR) markers in the target region,where the resistance gene in Kefeng No.1 was fine mapped to an 80-kb region containing six putative genes.Sequence and expression analyses of these genes revealed that SMV-resistance in Kefeng No.1 was probably attributable to three of the candidate genes(i.e.,Glyma.02 G127800,Glyma.02 G128200 and Glyma.02 G128300).Collectively,the results of this study will greatly facilitate the cloning of SC18-resistance genes and marker-assisted breeding of SMV-resistant soybean cultivars.更多还原