【作者】 孙玲伟； 戴建军； 张德福； 王锋； 姚惠娟；

【Author】 SUN Ling-wei;DAI Jian-jun;ZHANG De-fu;WANG Feng;YAO Hui-juan;Institute of Animal Science and Veterinary Medicine,Shanghai Academy of Agricultural Sciences;Division of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agri-genetics and Breeding;Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry,Nanjing Agricultural University;

【机构】 上海农业科学院畜牧兽医研究所； 上海农业遗传育种重点试验室动物遗传工程研究室； 南京农业大学动物科技学院江苏省肉羊产业工程技术中心；

【摘要】 目的本试验以不同妊娠期健康湖羊为妊娠期动物模型,利用代谢组学技术探究母羊生理状态下血液代谢组变化规律。方法选取怀有双羔的妊娠母羊(n=8),自妊娠50 d至110 d,每隔20 d采集母羊颈静脉血液,记录体重和采食量。试验期间母羊日粮按照NRC(妊娠期绵羊营养需要量)进行配制。对妊娠50 d、70 d、90 d和110 d四个时间点(分别为T1,T2,T3和T4)的母羊血液样品进行代谢组学的一维氢谱核磁共振技术(1H-NMR)检测,从而比较不同时间点之间妊娠期母体的代谢变化。结果随妊娠时间的延长,母羊的体重和采食量差异显著(P <0.05),并随之增加。对代谢数据进行多元统计分析,主成分分析结果显示时间点T2和T3的代谢状态逐渐远离T1,且时间点T4与T1处的代谢状态相差最多。对~1H-NMR谱图识别到的36种代谢物进行差异分析,共检测出13种差异代谢物(P <0.05),这些代谢物主要与氨基酸代谢和脂类代谢相关。其中,母体血浆中首次检测到1-甲基组氨酸和丙二酸的变化。结论该结果表明代谢组学检测平台能够用于评估绵羊不同妊娠时间点的血液代谢状态。在湖羊妊娠期,体内氨基酸代谢和脂类代谢对满足其营养需求尤为重要。更多还原

【Abstract】 Objective In this experiment,healthy Hu sheep in different gestational periods were used as pregnant animal models.The objectives of this study were to explore the changes of blood metabolites of sheep under physiological conditions with metabolomic techniques.Methods Pregnant ewes carrying twin fetuses(n = 8) were selected at 35 days of gestation(d G).All ewes received 100% of National Research Council(NRC) requirements of all nutrients and energy during this experiment.At 50,70,90,and 110 dG,maternal plasma samples were collected and designated as one of four corresponding time points(T1,T2,T3,and T4,respectively).Maternal plasma samples were analyzed using 1 H-nuclear magnetic resonance spectroscopy to compare their metabolomic profiles among time points.We used multivariate pattern recognition to screen for different metabolites in the plasma of the ewes.Results The body weight and food intake of the ewes were significantly(P <0.05) different at the four time points,and increased with the passage of pregnancy time.The principal component analysis model results showed that the metabolic states at time points T2 and T3 moved gradually further away from that at T1 and were furthest away from that at T1 at time point T4.Among the different time points,there were thirteen significantly differential metabolites in the maternal plasma(P < 0.05).These metabolites were closely related to amino acid metabolism and lipid metabolism,which might occur at different time points in pregnant ewes.In particular,newly observed changes in 1-methylhistidine and malonate were the first such changes found in maternal plasma.Conclusions These results demonstrate that the metabolomics approach has value for evaluating metabolism in pregnancy with advancing gestation.In conclusion,during normal pregnancy in Hu sheep,related metabolites play an important role in amino acid and lipid metabolism for meeting the nutritional demands of pregnant ewes.更多还原