【作者】 杨波； 王文； 毕磊； 曾清如； 周细红； 刘爱军；

【Author】 Yang Bo;Wang Wen;Bi Lei;Zeng Qingru;Zhou Xihong;Liu Aijun;Zhai Mingguo Academician Work Station, Sanya University;Key Laboratory of Environmental Nanotechnology and Health Effects Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences;College of Resource and Environment, Hunan Agricultural University;

【机构】 三亚学院翟明国院士工作站； 中国科学院生态环境研究中心环境纳米技术与健康效应重点实验室； 湖南农业大学资源环境学院；

【摘要】 采用经典的稀释混合平板法,从昆明滇池沉积物中分离出1株高效聚磷菌,并结合16sRNA基因序列分析进行了菌株的鉴定,在此基础上研究了其在不同理化因素条件下的除磷特性。研究结果表明,分离出的菌株为节杆菌属(Arthobacter sp.),将其命名为Arthobacter sp. B。在富磷培养基中接种培养12 h该菌株即能完成对数增长期,在磷浓度为2 mg/L、温度为30～35℃、pH值为6～7、以蔗糖、葡萄糖或麦芽糖作为唯一碳源和以牛肉膏、蛋白胨为唯一氮源时,菌株B在培养周期内生长迅速,体系的除磷率都在80%以上,而当温度低于15℃或高于35℃、pH值低于5或高于8时,菌株的生长受到明显抑制,除磷率也较低。同时研究结果发现,该菌株的生长会影响其生长环境基质的pH值,在一定范围内,可调节pH至其最适的生长区间。进一步的分析表明,该菌株利用碳源和氮源的方式和机理有别于传统的活性污泥中的反硝化聚磷菌,对蔗糖、葡糖糖等大分子有机碳源具有较高的利用率和亲和力,此外,该菌株不能利用铵盐作为唯一氮源来加以利用。更多还原

【Abstract】 A phosphate accumulating organisms was isolated from eutrophic sediment which located in Dianchi Lake, Yunnan, China. The strain, named Arthobacter sp. B, was obtained by classic methods for screening microorganisms together with 16 s rRNA gene analysis. Effects of different culture conditions to the bacterial growth and phosphorus removal efficiency were studied. The results showed that the logarithmic growth phase was completed within 12 hours after incubation, the optimal temperature range was from 30 ℃ ～ 35 ℃, the optimal initial pH was 6 ～ 7, and the best carbon source were sucrose, glucose or maltose, the best nitrogen source were beef extract or peptone. Under these optimized condition, the phosphorus removal efficiency was higher than 80% with maximum growth rate of the strain B. On the contrary, when the temperature was below 15 ℃ or above 35 ℃ and the pH was below 5 or above 8, which would obviously inhibit the growth of strain B, eventually led to lower phosphorus removal efficiency. The results also showed that the strain B could affect the pH during its growth period in a certain extent, and adjusting the pH value to its optimum range was observed. The utilization mode and mechanism of carbon source and nitrogen source for the strain was different from denitrifying phosphate accumulating organisms which existed in activated sludge. Further study revealed that the strain B could utilize macromolecule carbon source such as sucrose, glucose and maltose effectively. In addition, ammonium salt could not use be the sole nitrogen source to support the growth of strain B.更多还原