【作者】 何艺欣； 张兆雪； 何强； 李宏；

【Author】 Yixin He;Zhaoxue Zhang;Qiang He;Hong Li;Key Laboratory of Eco-Environment of Three Gorges Region,Ministry of Education,Chongqing University;

【机构】 重庆大学三峡库区生态环境教育部重点实验室；

【摘要】 以铜绿微囊藻(Microcystis aeruginosa)为研究对象,从蛋白质组学的角度,研究不同K+浓度(0、0.23、0.46和0.92mM)对铜绿微囊藻产毒能力的影响。结果表明,K+缺失条件下,胞外藻毒素浓度比对照组下降86%,产毒基因的相对转录丰度显著降低,而K+过量条件对产毒能力无明显影响;不同K+浓度培养下,铜绿微囊藻细胞中ABC转运体和光合作用相关的蛋白质的表达量受到明显调控,其中K+缺失对ATP的产生和主动转运过程有负面影响;由此推测,K+缺失条件下铜绿微囊藻产毒能力的降低,是由K+缺失所引起的产毒基因相对转录丰度的降低、光合磷酸化ATP供应不足及Fe3+主动转运受限所共同导致的.更多还原

【Abstract】 Microcystis aeruginosa was used as the research object to study the effects of different K+ concentrations（0,0.23,0.46 and 0.92 mM） on the toxicity of Microcystis aeruginosa from the perspective of proteomics.The results showed that with the absence of K+,the concentration of extracellular microcystins was 86% lower than that of the control group,and the relative transcriptional abundance of the mcy gene was significantly decreased,while the K+ excess condition had no significant effect on the toxin production ability of Microcystis aeruginosa.Under different K+ concentrations,the expression levels of ABC transporters and photosynthesis-related proteins in Microcystis aeruginosa cells were significantly regulated,and K+ abscence had a negative effect on ATP production and active transport processes.It is speculated that the decrease in the toxin-producing ability under K+deficiency was caused by the decrease of the relative transcriptional abundance of the mcy gene,the insufficient supply of photosynthetic phosphorylated ATP and the restricted transfer of Fe³⁺.更多还原