【作者】 胡玲萍； 张鸿伟； 张晓梅； 张恬恬； 常耀光； 赵雪； 徐杰； 薛勇； 李兆杰； 王玉明； 薛长湖；

【Author】 Hu Lingping;Zhang Hongwei;Zhang Xiaomei;Zhang Tiantian;Chang Yaoguang;Zhao Xue;Xu Jie;Xue Yong;Li Zhaojie;Wang Yuming;Xue Changhu;College of Food Science and Engineering,Ocean University of China;Technical Center of Inspection and Quarantine,Shandong Entry-Exit Inspection and Quarantine Bureau;

【机构】 中国海洋大学食品科学与工程学院； 山东出入境检验检疫局技术中心；

【摘要】 对虾贴错标签和掺假现象时常发生由于它们种间的相似性以及在加工过程中甲壳的脱除。为了区分三种十足目的商业虾种(日本对虾,中国对虾,南美白对虾),超高效液相色谱-飞行时间质谱((UPLC-QTOF)结合全部理论碎片离子的连续窗口采集(SWATH)用来检测所有可检测的胰蛋白酶消化的多肽。采用正交偏最小二乘判别分析(OPLS-DA)寻找潜在的生物标志物,BLAST用于物种特异性分析。随后,采用多反应监测(MRM)方法对多肽的灵敏度和选择性进行筛查,27条多肽被确定为最终生物标志物用于快速、准确区分对虾的物种而不需要高分辨质谱也不需要建立模型。这一策略页可以应用在其他含有高度同源的蛋白质产品认证。更多还原

【Abstract】 Incorrect labelling and adulteration of shrimp occurs due to interspecies similarities and carapace removal during processing.This study attempted to identify three related commercial shrimp species of the order Decapoda:Marsupenaeus japonicus,Fenneropenaeus chinensis,and Litopenaeus vannamei.Ultra-high-performance liquid chromatography coupled to high-resolution quadrupole time of flight mass spectrometry(UPLC-QTOF) with sequential window acquisition of all theoretical fragment ion spectra(SWATH) was used to discover all measurable trypsin-digested peptides in the individual shrimps.Further analysis of peptide biomarkers was carried out with an orthogonal partial least squares discriminant analysis(OPLS-DA) model.BLAST was used for species-specific analysis.Subsequently,multiple reaction monitoring(MRM) methods were developed for sensitivity and selectivity screening of the selected peptides,and 27 were identified as biomarkers allowing rapid and accurate discrimination of shrimp species without high-resolution mass spectrometry or statistical model building.These strategies could be applied in authentication of other products containing highly homologous proteomes.更多还原