【作者】 刘斌； 李昕； 任发政； 李媛；

【Author】 Liu Bin;Li Xin;Ren Fazheng;Li Yuan;College of Food Science and Nutritional Engineering,China Agricultural University;Beijing Advanced Innovation Center for Food Nutrition and Human Health;

【机构】 中国农业大学食品科学与营养工程学院； 北京食品营养与人类健康高精尖创新中心；

【摘要】 近年来类胡萝卜素、多酚黄铜类、活性肽和功能核酸等功能因子在癌症和代谢综合症具有较好的干预效果。然而这些敏感型活性因子存在的溶解性差、稳定性差和生物利用率低的问题限制了其在功能食品中的应用。最新研究报道纳米自组装作为一种新型的活性因子包埋技术可以显著地解决上述瓶颈问题。本实验室以特异性部分酶解为蛋白改性手段,成功地获得了能够自组装成粒径均一20纳米的两亲性多肽自组装载体。研究表面该蛋白纳米载体对于多种疏水性活性因子(槲皮素、虾青素、姜黄素和白藜芦醇等)均具有较高的包埋率。同时,经包埋之后的脂溶性活性因子的水溶性和胶体稳定性得到了提高。并且,经包埋之后的活性因子的生物活性和稳定性有显著提高。同时,所设计的载体还可以快速渗透小肠粘液层,克服粘液层屏障阻碍。并且,通过Caco-Ⅱ细胞模型发现纳米颗粒可以提高功能因子的细胞摄取和跨膜转运效率。同时利用Caco-Ⅱ细胞内抗氧化模型发现纳米颗粒可以提高功能因子的抗氧化能力。小动物活体实验发现半乳糖修饰的纳米载体具有优异的肝靶向递送白藜芦醇的效果,极大提高了对脂肪肝和胰岛素抵抗的干预效果。同时我们还构建了氧化多糖水凝胶-蛋白纳米载体的复合载体,提高了蛋白载体的胃肠道消化稳定性以及和粘液层的黏附性,增强了纳米载体的靶向缓释持效性。结果表明该复合载体在干预结肠炎和结肠癌以及益生菌定植方面发挥重要作用。我们的研究成果对于开发高生物利用率和高靶向性的功能因子食品奠定理论基础。更多还原

【Abstract】 The stability of the sensitive food bioactives has an important impact on their bioavailability and application.It is important to explore a novel method to protect and remain their bioactivity,and increase their dispersibility in food.Nanoscaled self-assembled biopolymers based on food grade materials are desirable in food application due to its biocompatibility and biodegradability.Here curcumin,resveratrol and astaxanthin are used as the model for hydrophobic bioactives.We have developed the self-assembled nanocarriers based on enzymatically hydrolyzed whey proteins.We discovered that the aqueous solubility,the stability and the activity were greatly improved after encapsulation.We find the mucus-penetration capability has been improved.The cellular uptake,transmembrane permeability and the cellular anti-oxidant activity are improved as well by Caco-Ⅱ monolayer models and CAA cellular anti-oxidation activity models.Besides,we also designed RGD peptides conjugated oxidized starch nanoparticles,which greatly increase the cell uptake of the incorporated ingredients.Besides,the hydrogel-protein NPs systems have been developed to have an enhanced mucoadhesion and slowed release properties.This research may be beneficial for the application of the functional carrier system based on natural biopolymers for functional foods.更多还原