【作者】 马蕾； 许冠英； 唐新雨； 蔡明珠； 王颉； 陈红；

【Author】 Ma Lei;Xu Guanying;Tang Xinyu;Cai Mingzhu;Wang Jie;Chen Hong;College of Food Science and Technology,Hebei Agricultural University;Food Science and Human Nutrition,University of Illinois at Urbana-Champaign;

【机构】 河北农业大学； 美国伊利诺伊大学香槟分校；

【摘要】 山楂(Crataegus pinnatifida),在中国和其他亚洲国家已有2000多年的历史,既作为传统的药用植物,也作为食物成分。本研究的目的是研究山楂多糖提取物(HPS)的抗癌活性及相关分子机制。方法:将山楂进行蒸馏水提取,然后进行70%～85%乙醇沉淀并冻干。用质量浓度为125,250,500,1 000μg/mL的HPS处理人结肠癌细胞(HCT116)12 h。通过免疫荧光染色评估磷酸化AKT和Ki-67的蛋白表达。通过流式细胞术分析细胞凋亡。使用实时定量RT-PCR检查mRNA表达。结果:HPS处理细胞不同程度降低Ki-67蛋白染色,HPS处理显着降低P-AKT蛋白表达。HPS显着增加凋亡细胞的比例。用500,1 000μg/mL HPS处理的HCT116细胞显着增加caspase 3,caspase 7,caspase 8和caspase 9的表达。HPS还以剂量依赖性方式上调FADD,TRADD和TNFR1的mRNA水平。结论:ECP通过诱导凋亡途径在HCT116细胞中表现出抗癌活性。结果表明,使用山楂提取物作为癌症患者的功能性食品的一些潜在益处。进一步的机制研究有必要建立与HPS的抗癌作用有关的分子途径。更多还原

【Abstract】 Crataegus pinnatifida,commonly named hawthorn,has been used in China and other Asian countries for more than 2,000 years,both as a traditional medicinal herb and as a food component.The objective of the study was to investigate the anti-cancer activities of extract from Crataegus pinnatifid(ECP) and related molecular mechanisms.Methods:The ground hawthorn was subjected to distilled water extraction,followed by 70%-85% ethanol precipitation,and lyophilization.Human colon cancer cell(HCT116) was treated with ECP at concentrations of 125,250,500,1000 μg/mL for 12 h.Phospho-AKT and Ki-67 expression were assessed by immunofluorescence staining.Cell cycle and apoptosis were analyzed by flow cytometry.mRNA expression was examined using real-time RT-PCR.Results:Ki-67 protein staining was reduced by ECP treatment in cells,and Phospho-AKT protein expression was significantly reduced with the ECP treatments.The proportion of apoptotic cells was significantly increased by ECP.Concomitantly,ECP treatment induced G1 phase arrest in HCT116 cells.HCT116 cells treated with 500 and 1000 μg/ml ECP had significantly increased expression of caspase 3,caspase 7,caspase 8 and caspase 9.ECP also upregulated the mRNA level of FADD,TRADD and TNFR1 in a dose-dependent manner.ECP downregulated the CyclinDl mRNA expression.Conclusions:Our findings demonstrated that ECP exhibited anti-cancer activities in HCT116 cells by induction of apoptosis pathway.ECP treatment also decreased proliferation by induction of Gl phase cell cycle arrest.The results indicate some potential benefits of using hawthorn extracts as functional foods for cancer patients.Further mechanistic studies are warranted to establish molecular pathways that are involved in the anti-cancer effects of ECP.更多还原