【作者】 郭学飞； 陈旭； 杨文胜； 刘长霞；

【Author】 Xuefei Guo;Xu Chen;Wensheng Yang;Changxia Liu;State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology;College of Life Science and Technology, Beijing University of Chemical Technology;

【机构】 北京化工大学化工资源有效利用国家重点实验室； 北京化工大学生命科学与技术学院；

【摘要】 ATP作为细胞的主要能源物质,其浓度及消耗速率是很多疾病,如帕金森症、某些恶性肿瘤等的指示信号,因此对ATP进行快速、灵敏检测具有重要意义^[1]。本工作通过热解二乙烯三胺五乙酸（DTPA）合成了尺寸约为2.3 nm的掺杂N的碳量子点（NCQDs）电致发光体,将该电致发光体NCQDs与ATP适配体作为电极材料构建电致化学发光（ECL）生物传感器来检测ATP。结果表明,ATP与其适配体的特异性结合导致ECL信号强度的增加,且ATP浓度在0-299 pM范围内与ECL强度呈现良好的线性关系（R²=0.998）,检测限为28.97 pM。该ECL传感器与其它基于ATP适配体的传感器相比,具有无标记、制备简单等特点,在ATP检测领域具有应用前景。更多还原

【Abstract】 Adenosine triphosphate(ATP) is generally acknowledged as the "energy currency" in cells, the concentration and dissipative rate of ATP are closely related to some common diseases, such as Parkinson‘s disease and some malignant tumors,so it is of great significance to detect ATP.In this work, N-doped carbon quantum dots(NCQDs) with dimensions about 2.3 nm were synthesized by the method of pyrolysis diethylenetriaminepentaacetic acid(DTPA).The NCQDs and ATP aptamers were used as electrode materials to construct the ATP biosensors by the means of electrochemiluminescence.The results showed that the specific binding of ATP to the corresponding aptamer led to an increasement in ECL signal intensity.The increasement of ECL intensity was found linearly to the concentration of ATP in the range of 0-299 pM(R~2=0.998) with a detection limit of 28.97 pM.Compared with other sensors based on ATP aptamer, this biosensor has the advantages of label-free and simple preparation, so it has a certain application prospect for ATP detection.更多还原