【作者】 王乾龙； 丁立生； 梁健； 谢静；

【Author】 WANGQianlong;DING Lisheng;LIANG Jian;XIE Jing;Chengdu Medical College;Chengdu Institute of Biology,Chinese Academy of Sciences;

【机构】 成都医学院； 中国科学院成都生物研究所；

【摘要】 采用直接竞争ELISA法实现了对橘子皮中残留2,4-D的检测。经过对该方法的验证,其线性范围为2～250 ng/mL(R~2=0.997),检测限为0.67 ng/mL,在该范围内相关性良好。其日内和日间的变异系数(coefficient of variation,CV)分别在3.12～7.47%和2.62～6.54%之间,均小于10%,精密度良好。平均回收率为96.7%,有较高的准确度和良好的特异性。因此,该直接竞争ELISA法可以快速准确的实现对橘子皮中残留的2,4-D检测。更多还原

【Abstract】 Using the direct competitive ELISA completes the detection of residual 2,4-dichlorophenoxyacetic in oranges peel.Results of the studies indicate that the linear range is from 2 to 250 ng/mL(R~2= 0.997) and the limit of detectionis 0.67 ng/mL.The precision of the method is good with the coefficient of variationsof intra-day and inter-day are from 3.12 to 7.47% and 2.62 to 6.54%,respectively,which were both low 10%.The specificity was satisfactory and the mean recovery was 95.5% so the precise was good.Therefore,this direct competitive ELISA could complete the fast and precise detection of residual 2,4-D in oranges peel.更多还原