【作者】 杨明； 雷力力； 张欣然； 张志国；

【Author】 YANG Ming;LEI Li-li;ZHANG Xin-ran;ZHANG Zhi-guo;The First Affiliated Hospital of Jiamusi University;

【机构】 佳木斯大学附属第一医院；

【摘要】 目的:建立测定人血浆中芬太尼浓度的高效液相色谱法。方法:本实验采用内标法,舒芬太尼为内标物。以正己烷-无水乙醇（95:5）为提取溶剂;以采用Sinochrom ODS-BP（4.6mm×200mm,5μm）色谱柱,甲醇-乙腈-0.13mol/L%乙酸铵水溶液（45:24:31）为流动相（PH=7.5）,流速为1.0m1·min^-1,柱温为室温,检测波长为215nm。结果:芬太尼的血浆浓度在31.25ng·m L^-1～1000ng·m L^-1范围内线性关系良好（r=0.9996）,最低定量限为31.25ng·m L^-1,方法回收率为94.2%～102.3%,萃取回收率70.5%～77.1%,日内、日间精密度均小于15%。结论:本方法操作简单、准确度和灵敏度高,适用于临床芬太尼血药浓度检测及药动学的研究。更多还原

【Abstract】 Objective: To determine the concentration of fentanyl in human plasma by HPLC. Method: The HPLC determination was performed by internal standard method.Sufentanil was the internal standard. The extraction solvent was n-hexane-absolute ethanol（95: 5）.The chromatographic separation was carried out on Sinochrom ODS-BP（4.6 mm× 200 mm,5μ m）column.The mobile phase consisted of solvent Methanol-acetonitrile-0.13 mol/L% aqueous ammonium acetate solution（45:24:31）（PH=7.5）.The flow rate was 1.0 m1·min^-1; the column temperature was set at room temperature and the UV detection wavelength was set at 215 nm. Results:The calibration curve for fentanyl was linear in the range of 31.25 ng·m L^-1～1000 ng·m L^-1（r=0.9996）. The lowest quantification limit was 31.25 ng·m L^-1; the methodological recovery was 94.2%～102.3% and the extraction recovery was 70.5%～77.1%. Both the intra-day and inter-day RSDs were less than 15%.Conclusion:The method was proved to be simple, accurate and sensitive, and it is applicable for determination of the trace concentration of fentanyl in human plasma and its pharmacokinetic study.更多还原