【作者】 褚福龙； 宋淑亮； 李云龙； 吉爱国；

【Author】 Chu Fulong;Song Shuliang;Li Yunlong;JI Aiguo;Marine College of Shandong University;Shandong University International Biotechnology Research and Development Center at Weihai;School of Pharmaceutical Sciences Shandong University;

【机构】 山东大学海洋学院； 山东大学威海国际生物技术研发中心； 山东大学药学院；

【摘要】 目的:本实验采用AKTA法来测定海带中提取的岩藻聚糖硫酸酯的含量。方法:采用AKTA法通过对岩藻聚糖硫酸酯的标准品进行测定,建立标准曲线,再使用同种色谱条件对实验室所提取的岩藻聚糖硫酸酯含量进行测定,并检验实验的精密度、准确度和稳定性。结果:实验表明此方法在岩藻聚糖硫酸酯浓度为0.2-1 mg/mL浓度范围内,线性关系较好,精密度、准确度、稳定性都较高。结论:通过对精密度、准确度和稳定性的测定,发现AKTA法测定岩藻聚糖硫酸酯含量结果较精确,进一步证明了AKTA法测定岩藻聚糖硫酸酯含量无论是原理上还是精度上都是完全可靠的。更多还原

【Abstract】 Aim: The content of the laboratory experiments to determine the fucoidan extracted from Laminaria japonica by AKTA method. METHODS:Based on the standard of determination of fucose to establish the standard curve by AKTA method, then the extraction of fucoidan content in laboratory using the same chromatographic conditions were determined, and the test precision, accuracy and stability. The experiment shows that this method is a good linear relationship, precision, accuracy, high stability. Through the determination of precision, accuracy and stability, AKTA method for the determination of fucoidan content results accurately, further proved that the AKTA method for the determination of fucoidan content either principle or accuracy is completely reliable.更多还原