【作者】 李宁； 邓建军； 朱巧英； 石艳艳； 成春岚； 倪雪梅； 罗红权；

【Author】 Li Ning;Deng Jianjun;Zhu Qiaoying;Shi Yanyan;Chen’s chun lan;Ni Xuemei;Luo Hongquan;Southwest Medical University;Department of Clinical Laboratory,Sichuan Maternal and Child Health Hospital;Department of Clinical Laboratory,Mianyang 404 Hospital;

【机构】 西南医科大学； 四川省妇幼保健院检验科； 四川绵阳四○四医院检验科；

【摘要】 目的:研究导流杂交基因芯片技术在检测人乳头状瘤病毒基因分型中的应用价值。方法:以2017年3月-2018年5月本科接诊且行生殖道人乳头状瘤病毒感染分型检测的病患3000例为研究对象,分析本组病患的检测结果,并对其亚型感染情况进行统计分析。结果:3000例病患检测出人乳头状瘤病毒阳性者710例,阳性率为23.67%(710/3000),当中有320例为高危亚型,有360例为低危亚型,有30例为中国人群比较常见型。人乳头状瘤病毒亚型中有420例为单一型感染,有290例为混合型感染。对于30-39岁组病患来说,其发生人乳头状瘤病毒感染的概率显著高于其它年龄组,组间差异显著(P<0.05)。结论:通过利用导流杂交基因芯片技术,可显著提高人乳头状瘤病毒基因分型的检测结果,为30岁及以上女性普查以及诊断宫颈病变的一种重要手段。更多还原

【Abstract】 objective:to study the application value of diversion hybrid gene chip technology in the detection of human papillomavirus genotyping.Methods:a total of 3,000 cases of patients who received undergraduate diagnosis and were tested for HPV infection by reproductive tract from March 2017 to May2018 were studied.Results:Of the 3,000 samples,710 were positive for human papillomavirus,with a positive rate of 23.67%(710/3000),among which 320 cases were high-risk subtypes,360 cases were low-risk subtypes,and 30 cases were common in Chinese population.Among the subtypes of human papillomavirus,there were 420 single infections and 290 mixed infections.The incidence of human papillomavirus infection was significantly higher in patients aged 30 to 39 than in other age groups,with significant inter-group differences(P <0.05).Conclusion:the detection results of human papillomavirus genotyping can be significantly improved by using the guidance hybrid gene chip technology,which is an important method for screening women aged30 and over and diagnosing cervical lesions.更多还原