【作者】 蒋书伦； 徐东坡； 方弟安； 赵长生； 杨健；

【机构】 南京农业大学无锡渔业学院； 水生生物资源研究室淡水渔业研究中心中国水产科学研究院；

【摘要】 为了探究氧化多环芳烃的毒性作用机制,以便了解其在水体环境中的污染危害,本研究采用半静水式接触实验法开展了9,10-菲醌对暗纹东方鲀96h的急性毒性研究,并使用代谢组技术对其血清中的代谢物进行了检测,与对照组对比分析后鉴定和筛选出了差异代谢物。结果显示,9,10-菲醌对平均体长(10.19±0.45)cm、平均体重(24.96±0.97)g的暗纹东方鲀96h的LC50为88.59μg·L-1;正模式下,9,10-菲醌与对照组之间在筛选出21个差异代谢物,主要与甘油磷脂代谢和苯丙氨酸代谢等通路有关;负模式下筛选出10个差异代谢物,主要与嘌呤代谢和硫代谢有关。这些通路主要通过干扰脂质合成、离子转运、氧化应激、细胞膜和神经递质功能障碍等代谢过程对暗纹东方鲀产生毒性影响。该研究使用代谢组方法探讨了氧化多环芳烃对暗纹东方鲀的毒性影响,为深入了解环境有机污染物对水体污染所造成的危害奠定了基础。更多还原

【Abstract】 In order to explore the toxic mechanism of oxygenated-PAHs, this study carried out an acute toxicity experiment on Takifugu obscurus with 9,10-phenanthrenequione. As the result revealed, the 96 h-LC₅₀ was figured out as 88.59μg·L^-1. In the positive mode,the present study totally identified and screened 21 differential metabolites compared to control group, which were mainly related to metabolic pathways such as glycerophospholipid metabolism, phenylalanine metabolism and so on, and 10 differential metabolites were screened in negative mode and mainly related to sputum metabolism and sulphur metabolism. These pathways exerted toxic effects on T. obscurus by disturbing metabolic processes such as lipid synthesis, ion transport, oxidative stress, membrane, neurotransmitter dysfunction and so on. This finding furnishes the deep understanding on toxic effects of oxygenated-PAHs with a metabonomic approach, and furtherly lays foundation for the assessment of environmental organic pollutants in water environment.更多还原