【作者】 乔慧； 许蕾； 江丰伟； 蒋速飞； 傅洪拓； 李菲；

【Author】 QIAO Hui;XU Lei;JIANG Fengwei;XIONG Yiwei;JIANG Sufei;FU Hongtuo;LI Fei;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture,Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;

【机构】 淡水渔业和种质资源利用重点实验室淡水渔业研究中心中国水产科学研究院； 南京农业大学无锡渔业学院；

【摘要】 日本沼虾通过减少身体活动和减少能量消耗来完成越冬。水温突然升高会导致蜕皮引发大量死亡,从而造成巨大经济损失。因此研究日本沼虾的蜕皮机制具有重要意义。蜕皮抑制激素在甲壳动物蜕皮过程中起着重要作用。本研究中,从眼柄克隆了日本沼虾蜕皮抑制激素的全长。Mn-MIH的总长度925bp,编码119个氨基酸。组织表达分析表明Mn-MIH在眼柄中表达最高,在鳃、卵巢和副神经节中几乎不表达。Mn-MIH对蜕皮周期的反应各不相同,这表明Mn-MIH对蜕皮发育有负调控作用。在长期干扰雄虾和雌虾后导致明显蜕皮周期加速,证明了Mn-MIH的蜕皮抑制作用。在雄虾长期干扰后,非雌虾的体重显著增加,确定了Mn-MIH在日本沼虾生长上的重要作用。更多还原

【Abstract】 In order to overwinter, M. nipponense displays decreased physiological activity and less consumption of energy. Sudden warming would trigger molting and cause an extensive death, resulting in huge economic losses. Therefore, it is of great practical significance to study the molting mechanism of oriental river prawns. Molt-inhibiting hormone gene(MIH) plays a major role in regulating molting in crustaceans. In this study, a full length MIH cDNA of M. nipponense(Mn-MIH) was cloned from the eyestalk. The total length of the Mn-MIH was 925 bp, encoding a protein of 119 amino acids. Tissue distribution analysis showed that Mn-MIH was highly expressed in the eyestalk, and that it had relatively low expression in gill, ovary, and abdominal ganglion. MnMIH varied in response to the molting cycle, suggesting that Mn-MIH negatively regulates ecdysteroidogenesis. Mn-MIH inhibition by RNAi resulted in a significant acceleration of molting cycles in both males and females, confirming the inhibitory role of MIH in molting. After long-term RNAi males, but not females had significant weight gain, confirming that MnMIH plays an important role in growth of M. nipponense.更多还原