【作者】 刘慧芳； 张莉娟； 林强； 刘礼辉； 梁红茹； 付小哲； 李宁求；

【Author】 LIU HuiFang;FU XiaoZe;ZHANG LiJuan;LIN Qiang;LIU LiHui;LIANG HongRu;LI NingQiu;Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences;

【机构】 中国水产科学研究院珠江水产研究所广东省水产动物免疫技术重点实验室； 上海海洋大学水产与生命学院；

【摘要】 活疫苗作为可浸泡式疫苗应用前景广泛,为了研制安全高效的针对鱼类弹状病毒的活病毒疫苗,本实验研究了三水加州鲈弹状病毒（MSRV-SS-7）疫苗株在鳜鱼体内的组织分布及消长规律,结果显示,MSRV-SS-7可在鳜鱼各组织中进行复制增殖,脑为主要靶器官,感染前期,在鳜鱼体内快速增殖,后期急剧减少直至检测不到;对不同种类鱼的安全性实验表明对鳜鱼、乌鳢、笋壳及加州鲈鱼均无明显致病力;毒力返强实验显示,鱼体盲传只在第一代感染的加州鲈鱼中检出MSRV-SS-7,无毒力返强现象。腹腔注射和浸泡免疫均可以为鳜鱼提供抵抗强毒鱼类弹状病毒的免疫保护,最小免疫剂量建议为10^3.67TCID50。疫苗冻干粉保存期为4℃保存7个月,免疫相关因子检测试验表明MSRV-SS-7不促进宿主Ⅰ-IFN途径相关基因的表达,但可诱导特异性抗体IgM的上调表达。本研究证实该弱毒疫苗株MSRV-SS-7具有良好的免疫原性和安全性,为鳜弹状病毒病的预防提供了理论依据和实验基础。更多还原

【Abstract】 Live vaccines have been widely used as immersible vaccines. In order to develop a safe and efficient live virus vaccine against fish rhabdovirus, this experiment investigated the MSRV-SS-7 distribution and change regularity in different tissues of Chinese perch, the results showed that MSRV-SS-7 could replicate in different tissues and had certain organ targeting in Chinese perch. MSRV-SS-7 was rapidly proliferated in the first 3 days post injection, but sharply decreased on the 4 th day post injection. The safety test of different kinds fish of MSRV-SS-7 indicated that MSRV-SS-7 had no obvious pathogenicity for Chinese perch, snakehead fish（Channa maculate）, marbled sand goby（Oxyeleotris marmoratus） and Largemouth bass（Micropterus salmoides）. The result of blind passage in fish indicated that the MSRV-SS-7 couldn’t be reversed to the virulent strain in the process of continuous infection because MSRV-SS-7 was only detected in the first generation of infected Micropterus salmoides. The immune efficacy tests of different immunization methods suggested that MSRV-SS-7 could provide protection for Chinese perch by immersion and injection immunization against virulent SCRV infection. Based on the results of challenge test, the minimum vaccination dose was 10^3.67 TCID50. The storage life of lyophilized vaccine powder was 7 months at 4 by the viral titer surveillance. The immune genes expressions results showed that MSRV-SS-7 could not enhance the typeⅠIFN-dependent genes expression, but motivate the IgM gene expression. This study confirmed that the attenuated vaccine strain MSRV-SS-7 has good immunogenicity and safety, and provides a theoretical basis and experimental basis for the prevention of fish rhabdovirus disease.更多还原