Toxicity and Secondary Metabolites studies of an Endophytic Fungus Isolated from the Fruit of Taxus chinensis

【作者】 吴汉夔； 申恒兰； 许世伟； 程颖； 贡俊苗； 季珂钦； 李佳琦； 王俊可； 伊青艺； 段宁慧； 孙凯； 邓德华；

【机构】 安阳师范学院化学化工学院；

【摘要】 Taxus chinensis is a species of yew. This plant is used against cancer, because paclitaxel or taxol can be produced from the bark of this plant. Due to the very limited plant resource available for the production of anticancer agents, our research is focusing on the microbes associated with the plant, which might produce possible anticancer compounds or taxol-like molecules. Therefore, endophytic microbes in the fruit of T. chinensis were studied, and one strain was isolated and the toxicity and secondary metabolites have been studied. Herein, we report the brine shrimp toxicity assay and chemical structures of the identified compounds. The crude ethyl acetate extract of the microbes cultured on rice media for 45 days at room temperature was tested for brine shrimp lethality in order to detect potential sources of novel cytotoxic, antitumor and pesticidal compounds. As a result, it showed quite potent cytotoxic activity with LC50 of 0.01mg/mL. Throughout repeated chromatography purification on Sephadex LH-20 packed column, as well as preparative HPLC, six compounds including streptomycinic acid(1) [1], alternator(2), clavocyrosolone(3), streptozotocin(4) [2], altenuene(5) and phialophopyrone(6) [3-4] have been isolated, and their chemical structures were elucidated on the basis of extensive analyses of the 1 H NMR and 13 C NMR, HMQC and HMBC data.更多还原

【Abstract】 Taxus chinensis is a species of yew. This plant is used against cancer, because paclitaxel or taxol can be produced from the bark of this plant. Due to the very limited plant resource available for the production of anticancer agents, our research is focusing on the microbes associated with the plant, which might produce possible anticancer compounds or taxol-like molecules. Therefore, endophytic microbes in the fruit of T. chinensis were studied, and one strain was isolated and the toxicity and secondary metabolites have been studied. Herein, we report the brine shrimp toxicity assay and chemical structures of the identified compounds. The crude ethyl acetate extract of the microbes cultured on rice media for 45 days at room temperature was tested for brine shrimp lethality in order to detect potential sources of novel cytotoxic, antitumor and pesticidal compounds. As a result, it showed quite potent cytotoxic activity with LC50 of 0.01mg/mL. Throughout repeated chromatography purification on Sephadex LH-20 packed column, as well as preparative HPLC, six compounds including streptomycinic acid(1) [1], alternator(2), clavocyrosolone(3), streptozotocin(4) [2], altenuene(5) and phialophopyrone(6) [3-4] have been isolated, and their chemical structures were elucidated on the basis of extensive analyses of the 1 H NMR and 13 C NMR, HMQC and HMBC data.更多还原